RE: [Histonet] Microscope filters

From:Margaret Blount

Dear Geoff,

Thanks, that is a great help. My colleague isn't actually using film as he
has a digital camera, but I will do a s you suggest and contact the
microscope manufacturers for trial samples. I can easily pop into Jessops
any time.

Thanks again


-----Original Message-----
From: Geoff McAuliffe []
Sent: Thursday, July 22, 2004 7:44 PM
To: Margaret Blount
Cc: Histonet (E-mail)
Subject: Re: [Histonet] Microscope filters

Hi Margaret:

    Assuming your colleague is using black and white negative film, a 
blue filter will pass more blue light to the film and give more density 
in the negative. When printed, that will translate to the blue subject 
being lighter on the print. That said, there are lots of blue filters 
used in photography and some trial and error may be needed. There are 
lots of different "daylight filters", depending on the light source and 
the amount of correction needed, an 80A filter might be blue enough for 
your application. The standard blue filter for making RGB separations is 
a #47 and it is a fairly dense blue-purple color. A #46 is very similar. 
Such filters are sold in photo stores that cater to professional 
photographers (Jessop's in the UK is one) but you might be able to find 
something in the AudioVisual dept at your institution or perhaps the 
manufacturer of your microscope might loan you several for a trial, you 
could then purchase the one that fits your needs.


Margaret Blount wrote:

>Hi all,
>I have a chinese colleague who requires a blue filter to suppress staining
>for Nissl substance (violet) in brightfield transmitted light microscopy -
>does anyone know what filter this is and a supplier of such a filter. So
>I have only identified a Daylight filter, but I am not sure if this would
>the trick or not. If anyone knows anything about this I would be very
>grateful as would my colleague.
>Thanks in anticipation.
>Margaret Blount
>Chief Technician
>Clinical Biochemistry
>University of Cambridge
>Addenbrooke's Hospital
>Hills Road
>CB2 2QR 
>Histonet mailing list

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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