[Histonet] Autofluorescence from RBC

From:"Randolph-Habecker, Julie"

Hey folks,

We are doing immunofluorescence on some paraformaldehyde-fixed
paraffin-embedded mouse lung that has been subjected to chemical injury. As
a result there is a great deal of hemorrhage. We have been using some
techniques to reduce autofluorescence from a paper suggested on histonet
(Baschong et al, JHC 49: 1565, 2001) however, the RBC are still very
fluorescent. Any other suggestions?????



Julie Randolph-Habecker, Ph.D.
Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave. N, G1-300
PO Box 19023
Seattle, WA 98109-1024
Tel: (206) 288-1187
FAX: (206) 288-1345

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