Tissue in OCT - how to "Thaw??? HELP!!
Hello Histonetters/Colleagues,
I have a piece of 'Pouch Young' (Wallaby) tissue approx. 1mm. in size that was fixed in 4% PFA then frozen in OCT. I now want to do 'WHOLE MOUNT IMMUNO. & am wondering what procedure IS best to 'thaw' the tissue with MINIMAL damage to the cells - OR anyone with previous experience RE: this situation can offer advice. Thankyou in advance,
Gratiana ( g.wijayanti@pgrad.unimelb.edu.au )
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