Tissue in OCT - how to "Thaw??? HELP!!

From:Bruce Abaloz

Hello Histonetters/Colleagues, 

I have a piece of 'Pouch Young' (Wallaby) tissue approx. 1mm. in size that was fixed in 4%  PFA then frozen in OCT. I now want to do 'WHOLE MOUNT IMMUNO. & am wondering what procedure IS best to 'thaw' the tissue with MINIMAL damage to the cells - OR anyone with previous experience RE: this situation can offer advice. Thankyou in advance,

Gratiana ( g.wijayanti@pgrad.unimelb.edu.au )
BRUCE ABALOZ                           PH:61383446282
HISTOLOGIST                            FAX:61383447909 
DEPT.of ZOOLOGY                        EMAIL: brucea@unimelb.edu.au
VICTORIA.AUSTRALIA 3010           

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