Re: RE: Processor failure

From:Jose Luis Palazon Fernandez

Not so long ago somebody in the list posted an abreviate process for reprocessing bad processed tissue. Maybe this would be less time consuming and probably will work with your samples. 

Jos#233# Luis

El dia 17/07/2003 01:37 usted envio el siguiente mensaje:
>Date: 17 de Julio de 2003 01:37:50
>From: "Favara, Cynthia (NIH/NIAID)"  
>Subject: RE: Processor failure 
> Processor failure
> What a 
> nightmare!!
> #160#
> I would reverse process 
> ie. paraffin x 4, xylene x 2, 100% ETOH x 2, 95%ETOH x 2. Then put it though 
> starting with the 95% ETOH to paraffin.
> #160#
> The time in the ETOH 
> already is likely to make tissue a bit brittle but should be able to get 
> readable sections.
> #160#
> Good 
> Luck!
> #160#
> Cynthia 
> Favara
>   -----Original Message-----
From: Joey Wilkie 
>   []
Sent: Wednesday, July 16, 2003 10:38 
>   AM
To: ''
Subject: Processor 
>   failure


Last week our embedded came in to embed and found 
>   that the tissue was not fixed.#160# After some investigation, we found that 
>   the some of the carbouys on the processors had been misplaced.#160# Our 
>   tissue had gone through a 10% formalin, a 100% alcohol , 50% alcohol , 80% 
>   alcohol , two 95% alcohols, 100% alcohol, back into a 10% formalin and then 
>   into two xylenes, and then into four paraffin baths.#160# We are using a VIP 
>   processor.

Now the question is how do we go about reprocessing 
>   the tissue.#160# Any help that you can give us would be greatly 
>   appreciated.

Thanks, Joey 
>   Mary's Hospital 
Grand Junction, 
>   Colorado 
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