RE: Warthin Starry microwave

From:Tony Henwood

Deb,
I believe that the original WS technique did not use a sensitiser. Later modifications (eg Steiner's) introduced a sensitiser to decrease background staining. Replacement sensitiser include Zinc Formalin, Lead nitrate and ferric alum (I may be in error on the last one- I will try it out).
 
Regards
 
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

 
 
-----Original Message-----
From: WWmn916@aol.com [mailto:WWmn916@aol.com]
Sent: Thursday, 10 July 2003 11:30
To: histonet@pathology.swmed.edu
Subject: Warthin Starry microwave

Greetings everyone,
Say, has anyone tried the microwave Warthin Starry method that is in Freida Carson histology book?  I was successful in getting spirochete staining, but am wondering if since we can't use uranyl nitrate in our lab (which is why this particular procedure was appealing to try out) if some certain spirochete types will only stain using uranyl nitrate?  The control piece of tissue I worked up this method with stained positive and without the uranyl nitrate.  I need to know before I put this out to our doctors, whether or not there are some types of spirochetes that can only be demonstrated with uranyl nitrate.  Thanks for any help.

Deb King, HT
Sacramento, CA


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message and any attachments are those
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any
consequential damage resulting from email containing computer
viruses.
**********************************************************************

<< Previous Message | Next Message >>