RE: Warthin Starry microwave

From:Tony Henwood

I believe that the original WS technique did not use a sensitiser. Later modifications (eg Steiner's) introduced a sensitiser to decrease background staining. Replacement sensitiser include Zinc Formalin, Lead nitrate and ferric alum (I may be in error on the last one- I will try it out).
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: []
Sent: Thursday, 10 July 2003 11:30
Subject: Warthin Starry microwave

Greetings everyone,
Say, has anyone tried the microwave Warthin Starry method that is in Freida Carson histology book?  I was successful in getting spirochete staining, but am wondering if since we can't use uranyl nitrate in our lab (which is why this particular procedure was appealing to try out) if some certain spirochete types will only stain using uranyl nitrate?  The control piece of tissue I worked up this method with stained positive and without the uranyl nitrate.  I need to know before I put this out to our doctors, whether or not there are some types of spirochetes that can only be demonstrated with uranyl nitrate.  Thanks for any help.

Deb King, HT
Sacramento, CA

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