sectioning fresh prostates

From:"Charles W. Scouten, Ph.D."

Question:We are interested in obtaining fresh tissue from radical
prostatectomy specimens. For this we need to slice 2-3mm sections of the
fresh prostate which is a rubbery organ measuring approximately 5x5x5cm
(it can be somewhat smaller or larger). It is important to obtain whole
slices that contain the entire dissection margin of the organ. The
problem is that without fixation in formalin there is distortion of the
tissue after sectioning so a really sharp knife and a way to hold the
firmly is needed. Do you have a solution for this?

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