RE: CAP/cryostat

From:"Tague, Curtis"

I use 100% alcohol to prevent against freezing. Usually do that on a Friday and allow it to dry completely over the weekend.

 -----Original Message-----
From: 	Paula Wilder [mailto:histo20@hotmail.com] 
Sent:	Tuesday, July 08, 2003 10:09
To:	histonet@pathology.swmed.edu
Subject:	

Hello everyone!

I was just looking over the CAP recommendations for routine decontamination 
of the cryostat.  It suggests cleaning regularly with 70% alcohol and 
decontaminating with a tuberculoidal disinfectant weekly.  We do 
approximately 30-50 frozens weekly.  My question - would the 70% alcohol 
need to be followed by acetone to prevent freezing?  Does anyone 
decontaminate their cryostats weekly - the older models would require an 
excessive amount of work?  We have a Shandon model as a backup which uses 
formalin for fumigation, so that would not pose such a problem.  Any help in 
this would be greatly appreciated.  Thanks so much!
Paula Wilder
St. Joseph Medical Center
Towson, MD 21204

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