Counterstain for NBT/BCIP

From:Gayle Callis

We use nuclear fast red, and make it in house.  Unfortunately, some
commercial solutions have proven inferior for the job (you might have a
good source of ready-to-use NFR).  If you use inhouse prepared solution,
you may need to back off staining time - 10 dips or so works well for us.
The red against the very dark blue/black chromogen is outstanding. 
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


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