I've read about the Acid-haematin technique being one of the best techniques
for staining mitochondria. The procedure asks for frozen sections of
formal-calcium fixed material. Originally this technique is used for choline
contaning phospholipids. So what I'd like to know, is the formal-calcium
fixation and frozen sections only required if you are using the procedure to
demonstrate phosphatidyl cholines. But for the demonstration of mitochondria
these two requirements are not important. Is this the case, if not what
other histochemical techniques demonstrate mitochindria in formalin fixed
paraffin embedded material.



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