Good morning Histonetters - I have a question from a graduate student and 
having never done this technqiue I am at a loss.  The graduate is trying 
to do a fluorogold technique on ganglia.  She is cutting frozen 
sections.  The sections are terrible (I will help her there), but they 
consistently wash off.  
     Question:  Should the tissues be put on coated slides, Probe-On 
slides, charged slides, etc. to help the tissue stick or will that 
interfere with the fluorgold deposition?  
     Any help you can offer will be greatly appreciated.  Cheryl


Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Baton Rouge, LA  70803

(225) 578-9784
FAX  (225) 578-9720