Have you tried aqueous eosin with hematoxylin.  The eosin will fluoresce under
UV light and the hematoxylin will produce a dark background.
Patsy

Frank Rosenthal wrote:

> Does anyone have suggestions for a high contrast (LM) stain for alveolar
> boundaries in air-dried (inflated) lung tissue embedded in glycol
> methacrylate.   I have tried H&E, Toluidine Blue, Methylene Blue and
> Polysciences Multiple Stain with limited success.  The high contrast is
> needed for proper processing in an image analysis system.  Any suggestions
> or ideas will be very much appreciated.
>
> Thanks.
>
> *****************************************
> Frank S. Rosenthal, Ph.D.
> Associate Professor
> Purdue University School of Health Sciences
> 1338 Civil Engineering Building
> West Lafayette, IN 47907 USA
> tel: 765-494-0812, fax: 765-496-1377,
> e-mail: frank@purdue.edu
> *****************************************