Dear Frank:

    Hmmmm. If the cell coats will stain with PAS that coupled with the
appropriate filter/software might to the trick. Or a PA silver methenamine
which gives a black rxn. product.Otherwise how about phase contrast
illumination or differential interference/Nomarski/Hoffman modulation contrast
illumination?

Frank Rosenthal wrote:

> Does anyone have suggestions for a high contrast (LM) stain for alveolar
> boundaries in air-dried (inflated) lung tissue embedded in glycol
> methacrylate.   I have tried H&E, Toluidine Blue, Methylene Blue and
> Polysciences Multiple Stain with limited success.  The high contrast is
> needed for proper processing in an image analysis system.  Any suggestions
> or ideas will be very much appreciated.
>
> Thanks.
>
> *****************************************
> Frank S. Rosenthal, Ph.D.
> Associate Professor
> Purdue University School of Health Sciences
> 1338 Civil Engineering Building
> West Lafayette, IN 47907 USA
> tel: 765-494-0812, fax: 765-496-1377,
> e-mail: frank@purdue.edu
> *****************************************

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************