I am trying to work up Zymed's e-cadherin on our NexES without much luck. I am getting a lot of cytoplasmic staining (nonspecific) and not very much membrane staining. Help!
I have tried many things, but am having "better" results with a 1:100 dilution at 32 min. and steaming it in EDTA. I am testing it on different breast CA's
Fairview-University Medical Center
Mpls. MN 55454
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