I am starting a new project immuno staining fixed paraffin embedded rat
brain. I have worked with human brain and had no control of the fixation.
My experience is limited on working with rat brain and am looking for any
guidance with the fixation step. I am inclined to fix w/ 10% NBF. I have
done this in experimenting with one mouse brain and it came out great. But
I am looking for the optimal fixation for rat brain. The tissue must be
paraffin embedded, so fresh is out. I will be doing H&E, immuno stains, and
Apotag(in-situ).  Any suggestions out there?

Kim Carter HT
Ohio State 

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