Do IHC sequentially. The first one is with a permanent chromogen (DAB,
BCIP), then place slides in a chaotrophic buffer at elevated temperature (we
microwave slides at 10sec/slide in a Retrievit-8 buffer). Wash and proceed
with the second IHC, starting from the protein blocking step.
> From: Sunil Thomas K [mailto:email@example.com]
> Sent: Monday, July 23, 2001 6:25 AM
> To: Bruce Abaloz
> Cc: firstname.lastname@example.org
> Subject: Re: IHC Double staining
> --- Bruce Abaloz <email@example.com>
> can not find a protocol or a kit that would
> > allow me to achieve this with two antibodies raised
> > in the same species.
> I am also a student-my opinion is that, if you use
> primary antibodies raised in same species how will you
> know what the secondary antibody is recognising.
> The alternative is to use primary antibodies that are
> tagged to different labels. the specificity of such
> detection will be low.
> Sunil Thomas K
> > Histonetters,
> > I am a student in the department of Zoology at the
> > University of Melbourne, Australia. I have two
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