We use Leu-M1 from B/D with a 40 minute, 96 degree C. waterbath pretreatment and DAKO's EnVision+ detection.

R. Cartun

>>> ANN MARUSKA <amarusk1@FAIRVIEW.ORG> 06/27/01 04:43PM >>>
Hi Histonetters,

Just wondering what other labs are using for a CD15 antibody to detect Reed Sternberg cells.  Our CD15 has become very inadequate on our NexES - even with a heat retrieval.  Currently we are using Ventana's product (clone MMA).

Thanks in advance.



Ann Maruska
Fairview-University Medical Center
Mpls. MN  55454
amarusk1@fairview.org 
612-273-9119