undecalcified bone with muscle, etc

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu>
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polymethylmethacrylate (PMMA) is the method of choice.  It is toxic,
requires fume hoods, is time consuming, and for microtomy, a tungsten
carbide knife is required.  However, one can do thicker ground sections (70
- 100 um thick) cut on special saws, ground and polished followed by some
surface staining technics (these are actually easy to do, but not all
dyes/staining technics will work).

If she wants immunostaining, ground sections are not the best choice since
the MMA must be totally removed.   Microtomes need to be powerful enough to
section undecalcified bone with a tungsten carbide knife.

This is just the tip of the iceberg for setting up undecalcified bone
histotechniques, needs vacuum setups, plus other things.  

A suggestion, the NSH annual symposium convention in Milwaukee has several
bone histotechnic workshops this year, and one is on PMMA technics (Brown
and Hyten).  Would be a great way to access info/get started, as there are
few or not books written on how to do this.  The Journal of
Histotechnology, over the last 15 years or so, has several articles written
on PMMA including staining. Look for these authors, Sterchi, Eurell,
Sanderson, Chappard, Jenkins, Hand, and a review of bone in 1998 or 1999, a
review article by Cathy Sanderson (Mayton), that has tons of info, with
referencing to help you fill in gaps where a book is lacking.   

What does she want to see that requires plastic technic?  Just curious.  
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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