quenching autofluorescence
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From: | Mike King <making@nersp.nerdc.ufl.edu> |
To: | histonet@pathology.swmed.edu |
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Yesterday,"Barry, Lilith" <Lilith.Barry@nrc.ca> posted
Subject: Quenching autofluorescence
...Is there a way of quenching or blocking this autofluorescence before doing
the immuno? ...
In the Molecular Probes catalog there is a procedure for this, I don't have
the catalog at the moment but I believe it is simple immersion in 0.5%
sodium borohydride for a few minutes prior to antibody incubations. You
could test it and work out the necessary reaction times on sections not
being immunolabelled.
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