quenching autofluorescence

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From:Mike King <making@nersp.nerdc.ufl.edu>
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Yesterday,"Barry, Lilith" <Lilith.Barry@nrc.ca>  posted
Subject: Quenching autofluorescence
...Is there a way of quenching or blocking this autofluorescence before doing
the immuno? ...

In the Molecular Probes catalog there is a procedure for this, I don't have 
the catalog at the moment but I believe it is simple immersion in 0.5% 
sodium borohydride for a few minutes prior to antibody incubations.  You 
could test it and work out the necessary reaction times on sections not 
being immunolabelled.

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