Sectioning Cardiac Stents

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Dear Histonetters:

    Anyone out there in Histoland successfully producing sections of cardiac 
artery containing metal stents?  I have had some moderate success on past 
samples, but the most recent specimens have me frustrated.  The researchers 
want to be able to do histomorphometric measurements on the stained sections 
and I can't seem to get the intima's to lay flat without folds and wrinkles.  
The specimens are embedded in PMMA and cut on a Jung Model K microtome with a 
carbide tungsten blade.  I'm sure there are some "tricks" involved...any 

Thanks in advance!!


Diane Mahovlic, HT(ASCP)
Orthopedic Pathology Laboratory
The Cleveland Clinic Foundation
9500 Euclid Avenue
Cleveland, OH   44195

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