Re: whole mouse imaging in real time

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From:Abizar Lakdawalla <>
To:Gayle Callis <>
Content-Type:text/plain; charset=us-ascii

hi gayle, thanks for the reference! Though I think the approach would only work
with transgenic mice that have an introduced luciferase gene. Do you have more
details about the imaging set up?

Gayle Callis wrote:

> A group at Stanford uses a CCD camera and a luciferase-activating substrate
> to perform
> "Real time in vivo imaging of T cell trafficking in autoimmune disease"
> poster #149.23 at the Seattle 2000 FASEB meeting.  Sandora MR, Costa GL,
> Benson JM, Lejon K, Slavin AJ, Contag CH and Fathman CG.
> Hopefully they have publications which can be located via PUBMED describing
> this method in detail.
> This permits a systemic delivery of the substrate, and whole animal (live!)
> was imaged, which visualized all organs at the same time, defining the
> kinetics of T cell trafficking.
> The camera setup probably is considerably cheaper and smaller than a huge
> cryostat, Instrumedics Cryojane Tape Transfer setup, plus all the work of
> just cutting sections through a whole mouse.  PLUS a mouse could be tested
> (in vivo) at different time intervals, time and cost saving, versus
> adoptive transfer methods that require euthanasia of mice at time intervals
> (ex vivo).
> Very elegant!
> After pricing cryostats, a monster Leica or Polycut, lack of space for
> housing the instrument and all the pricey extras and just the time involved
> in serial or semi serial sectioning a mouse!!! we are looking into the CCD
> setup instead.
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717-3610
> 406 994-4705
> 406 994-4303

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