Re: Apoptosis in normal lung tissue

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From:Luis Chiriboga <>
To:Victoria Baker <>
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Try the TUNEL for comparison.
Regards Luis

Victoria Baker wrote:

> Hi All!
> I'm currently doing a study on apoptosis in mouse
> tissue the animals are being induced and treated in 6
> different groups.  Diet is also being put into the
> factor - high, moderate & low.  The pathologist here
> is concerned that our results are too low in the
> treated/induced mice.  Our thymus control shows normal
> results.  Our normal lung has maybe one or two,
> sometimes none.  In all the literature I have read I
> see that the results of normal lung tissue is very
> low, but no one give a number.  My understanding is
> that it can vary from 1-3 or even none.  Also I see
> that most everyone is looking not only at In-situ
> results, but at routine H&E as well.  What I'm trying
> to get is a better in sight as to what others in the
> field are doing to make a decision on apoptosis and
> what methods they are using to come to this
> conclusion.
> Thank you for any assistance/information anyone may
> have.
> Sincerely,
> Vikki Baker
> American Health Foundation
> Valhalla, New York
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Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
Bellevue Hospital
NYU School of Medicine
First Avenue & 27th Street
New York, NY 10016

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