Re: ER and Golgi
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From: | Kenneth Chapman <kchapman@mail.med.upenn.edu> |
To: | "J. A. Kiernan" <jkiernan@julian.uwo.ca>, "histonet@pathology.swmed.edu" <histonet@pathology.swmed.edu> |
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Hi John,
Thanks for answering! You did this staining on paraffin sections? Do you
have any information regarding protocols?
Ken
"J. A. Kiernan" wrote:
> On Fri, 28 Jul 2000, Kenneth Chapman wrote:
>
> > I am presently staining ER and Golgi on paraffin sections using
> > Molecular Probes Concanavalin A and Wheat Germ Agglutinin Conjugates
> > respectively. Has anyone ever done anything like this before?
>
> Yes. In the early 1970s I had classical Golgi apparatus staining
> with concanavalin A in neurons: a perinuclear distribution quite
> different from the rough ER (Nissl) staining of the same cells.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
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