Re: ER and Golgi

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From:Kenneth Chapman <>
To:"J. A. Kiernan" <>, "" <>
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Hi John,

Thanks for answering!  You did this staining on paraffin sections?  Do you
have any information regarding protocols?


"J. A. Kiernan" wrote:

> On Fri, 28 Jul 2000, Kenneth Chapman wrote:
> > I am presently staining ER and Golgi on paraffin sections using
> > Molecular Probes Concanavalin A and Wheat Germ Agglutinin Conjugates
> > respectively.  Has anyone ever done anything like this before?
>   Yes. In the early 1970s I had classical Golgi apparatus staining
>   with concanavalin A in neurons: a perinuclear distribution quite
>   different from the rough ER (Nissl) staining of the same cells.
>  John A. Kiernan,
>  Department of Anatomy & Cell Biology,
>  The University of Western Ontario,
>  LONDON,  Canada  N6A 5C1

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