RE: Microwave Frozen Section Stain
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From: | Steven Slap <SSlap@ebsciences.com> |
To: | "HISTONET@PATHOLOGY.SWMED.EDU" <HISTONET@PATHOLOGY.SWMED.EDU> |
Reply-To: | |
Content-Type: | |
Hi Histonetters
Daryl Mikita asked about the use of microwaves and frozen sections. There
is a chapter devoted to this topic ("Combining Microwave and Freeze
Techniques) in Kok and Boon's Microwave Cookbook for Microscopists. In
this chapter, Dr. Boon makes a case for a brief microwave fixation step
using an alcohol/polyethylene glycol fixative, Kryofix, prior to staining.
Dr. Boon concludes that "In our experience,, this method, this method has
proven to be rapid and reliable, enabling 'quick section' diagnosis. The
task of the pathologist is greatly facilitated when he is provided with
these excellent sections for his diagnostic work." A fast H & E method to
follow the Kryofix microwave fixation is also provided (p.217).
The other advantage of the microwave fixation method, beside improved
morphology, was preservation of immunoreactivity. Dr. Boon wrote "In a
pilot study...all antigens under investigation survived both short-term
ethanol <Kryofix> fixation and microwave heating to 50 degrees C. In all
cases, immunostaining of cryostat sections was excellent."
For further reading, Kennedy A Foulis AK (1989) Use of microwave oven
improves morphology and staining of cryostat sections. J Clin Pathol
42:101-105; Nakae et al (1990) Application of microwave irradiation in
surgical pathology; improvement of microscopic-image of cryostat sections
and exploration in rapid metallic stainings. Acta Histochem
Cytochem23:573-583.
Note: Kryofix was a Merck product and is no longer manufactured. Merck now
manufactures a similar fixative called Neofix and Energy Beam Sciences
sells a replacement fixative called Microfix.
Best regards,
Steven Slap
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The Laboratory Microwave Company
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