Carolyn:

Although various "citrate based" AR formulations work fairly well.  We have
found that Novocastra's 6F11 works much better when using a 0.1 M Tris-HCl
pH 9.  See Brancroft and Stevens for proper ratios of Tris Base and
Tris-HCl.

Hope this helps,

Carlos Genty
Breast Center Pathology
Baylor College of Medicine
Houston, Texas
cgenty@bcm.tmc.edu

-----Original Message-----
From: carolyn.luty@muhc.mcgill.ca [mailto:carolyn.luty@muhc.mcgill.ca]
Sent: Wednesday, July 19, 2000 1:56 PM
To: histonet@pathology.swmed.edu
Subject: ER




We are having problems with very weak staining with ER. We use antigen
retrieval
in citrate buffer in the microwave.The antibody was from Nova-Castra and
then we
tried one from Biogenex with the same results. Can anyone give any
suggestions
??.

Carolyn Luty
Montreal General Hospital