Re: TTF-1 and CKBeta34E

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From:Katri Tuomala <>
To:"Sanders, Julie, VHACIN" <>
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Hi Julie,
What are you using as a pre-treatment for your BE3412 antibody? I was not
successful either on prostate tissue using my regular trypsin protocol. I went
for HIER with citrate in a microwave and got good results, but at times also
nuclear staining and bleeding. Now I am using 0.05% protease (Sigma) in TBS pH
20 min. at room temperature and everybody is happy. Hope this helps.

Katri Tuomala
Anatomic Pathology
StJoseph's Hospital
Hamilton, Ontario, Canada

"Sanders, Julie, VHACIN" wrote:

> Good Morning All,
>    We are having problems with the TTF-1 on the Ventana.  I have spoken to
> the vendor of the antibody, and we have tried everything under the
> sun...longer antigen retrieval time, using BioCares Nuclear Decloaker,
> different TTF-1 from other vendors (all prediluted) to no avail...any
> suggestions?
>    Also, we do CKBeta34E on prostate, but prostate is not working as a
> control.  Skin works wonderfully, but our pathologists insist that we use a
> prostate control...again suggestions will be welcomed.
> Thanks in advance for the responses,
> Julie
> Julie A. Sanders, BA, HT(ASCP)
> Supervisor, Anatomic Pathology/Administrative Office
> VAMC, Cincinnati, Ohio

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