Hi Julie,
What are you using as a pre-treatment for your BE3412 antibody? I was not
successful either on prostate tissue using my regular trypsin protocol. I went
for HIER with citrate in a microwave and got good results, but at times also
nuclear staining and bleeding. Now I am using 0.05% protease (Sigma) in TBS pH
7.6,
20 min. at room temperature and everybody is happy. Hope this helps.
Katri

Katri Tuomala
Anatomic Pathology
StJoseph's Hospital
Hamilton, Ontario, Canada

"Sanders, Julie, VHACIN" wrote:

> Good Morning All,
>    We are having problems with the TTF-1 on the Ventana.  I have spoken to
> the vendor of the antibody, and we have tried everything under the
> sun...longer antigen retrieval time, using BioCares Nuclear Decloaker,
> different TTF-1 from other vendors (all prediluted) to no avail...any
> suggestions?
>    Also, we do CKBeta34E on prostate, but prostate is not working as a
> control.  Skin works wonderfully, but our pathologists insist that we use a
> prostate control...again suggestions will be welcomed.
>
> Thanks in advance for the responses,
> Julie
>
> Julie A. Sanders, BA, HT(ASCP)
> Supervisor, Anatomic Pathology/Administrative Office
> VAMC, Cincinnati, Ohio