On Tue, 27 Jun 2000, Peter Poon wrote:

> We have been using Vector's Nova red. but right now it's giving us color
> incompatiblity with the Ethyl Green/ Pyronin Y staining that we use as a
> counterstain.  So we are thinking of switching to DAB as the chromogen.
> Does anyone have any preferences to what type (brand or preparation)  of
> DAB they use?  Have anyone done Ethyl Green/Pyronin Y with any other
> chromogen that works well with this counter stain? If so what did you use?
> 
> 
> Thanks for all your help
> 
> 
>    __________________________________________ 
>    \    Peter Poon                           \     _         ______ | 
>     \   http://cafe.berkeley.edu/~peterp      \  /   \___-=0`/|0`/__| 
>      \  (510)649-9654                          \_\          / | /    ) 
>      /                                         /  `/-==__ _/__|/__ -| 
>     /                                         /   *             \ | | 
>    /                                         /                   (o) 
>    ------------------------------------------ 
> 
> 

Peter
I have been using a DAB kit from Dako, LSAB2 cat# K0675 with wonderful
results.  No background.  Very easy to use.  This is a large volume cat#
and they also have a smaller volume kit.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey
USA