RE: Re - formaldehyde substitutes

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From:"Petrilli, Michael" <Michael.Petrilli@med.va.gov>
To:'Marylou Pohl' <mbpohl@acsu.buffalo.edu>, Clarke Ian <clarke.ian@virgin.net>
Reply-To:
Content-Type:text/plain

We had problem with prefer-fixed tissue and immunos.....have discontinued
use.

> -----Original Message-----
> From:	Marylou Pohl [SMTP:mbpohl@acsu.buffalo.edu]
> Sent:	Sunday, June 25, 2000 1:27 AM
> To:	Clarke Ian
> Cc:	histonet
> Subject:	Re: Re - formaldehyde substitutes
> 
> We have partially gone to formalin substitute fixation using Prefer from
> ANATECH
> because my prosector is sensitized to formalin..  By using a Lipshaw Gross
> Lab
> Senior and using Preefer for the bx's, we have seemed to help her continue
> in
> her career.  By partially I mean that we only fix our biopsies in Prefer,
> but
> that it a lot.  This fixative is given out to the GI, urology and
> pulmonary
> clinics.  We do not use Prefer for dermatology specimens as it lyses red
> blood
> cells and that would hinder a vasculitis dx with derms.
> 
> Our larger specimens coming from OR still are 10%NBF fixed.  They are
> thoroughly
> rinsed prior to dissecting.
> 
> Hope that helps.
> 
> Clarke Ian wrote:
> 
> > Dear Histonetters,
> >                                 I have a query regarding the
> formaldehyde
> > substitutes ,Prefer,Preserve and glyco fixx .We are in the process of
> trying
> > out these alternatives and have been told by a Rep, that we would
> require
> > formaldehyde to store tissue samples .As we are wanting to eliminate the
> use
> > of formaldehyde in our laboratory is this the case?Does anyone out there
> > utilise these fixatives routinely and do you store your tissue in these
> > products .I know we could use 70% alcohol as a storage medium but this
> may
> > cause a fire risk.
> >
> > Thanking you in advance
> >
> > Ian Clarke
> > Histopathology/Cytopathology Department
> > Craigavon Area hospital NHS Trust
> > UK << File: Card for Marylou Pohl >> 



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