RE: Alcian Blue for ... [urban myths]
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Histonet <Histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Sat, 1 Jul 2000, jim wrote:
> I think that the concentration of Alcian Blue will soon be academic. ...
> ... ProSciTech has no more Alcian Blue available. "
This was aired on Histonet several months ago, and major
suppliers indicated that there was no impending shortage of
the dye. The Colour Index (CD-ROM, 1996) indicates that
it is still manufactured by 2 or 3 companies as a textile
dye, under various trade names. Any supplier of biological
stains can buy the textile dye, test it as a stain, and
sell it as alcian blue.
The Biological Stain Commission regularly tests batches
of alcian blue submitted by vendors and certifies them
if they are OK for staining. Basic copper phthalocyanine
dyes labelled alcian blue have always been pretty variable,
so it's best to buy from a certified batch.
There's nothing new in perceived shortages of biological
stains. In the early 1970s haematoxylin was supposedly in
short supply (= prices up) for such mythical reasons as a
disease of Carribean logwood trees and a sunk ship that
carried the world's harvest of Haematoxylon heartwood.
In the early 1990s there was a similar non-shortage of
light green SF (supposedly because of a banned toxic
compound used in its manufacture). The Biological Stain
Commission soon sorted that one out (Penney & Powers 1995
Biotechn Histochem 70:217). There was never a real
shortage of the dye, but some bogus compounds (not certified
by the BSC) had been sold, and they didn't work.
(Bear in mind also that fast green FCF is better than
light green in every way, and has identical staining
properties.)
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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