How can you successfully perform immunohistochemistry on slides of liver
tissue using horse-radish peroxidase and DAB?  How do you quench the
endogenous peroxidase in the tissue so everything doesn't turn brown?  I've
tried 3, 10 and 15 % H2O2 in both methanol and di water for 20 min. up to 1
hour with no success.  The tissues are formalin fixed and embeded in
paraffin.  We then used a pH 6.0 citrate buffer/microwave antigen retrieval
system for our primary Ab detection.  Even with all that the endogenous
enzyme caused the false positive with the DAB.  Anyone have any
suggestions?
Thank you for all replies.
Karen in Omaha