that sinking feeling

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From:"Karen D. Larison" <> (by way of histonet)
To:histonet <>
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Hello histonetters,

I have a graduate student who has done some physiology on a monkey brain.  He
now wants to cut cryosections of this brain (electrode tracks have been marked
with DiI), and has put a portion of the brain (maybe a 3-4" cube) in 30%
sucrose and is waiting patiently for it to sink.  He's been waiting for over a
week now.  My question is:  Do all brains sink in 30% sucrose, or are the
brains of "higher" organism too myelin-rich to sink?  Any responses will be
appreciated.  He defends in about 3 weeks, and is a bit nervous as this work
represents his last piece of supporting data.

Thanks for all your help.

Karen Larison - University of Oregon

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