I think  the cubes are too large. If he can make them smaller they will sink
faster.
Lilith

Lilith Ohannessian-Barry
National Research Council
Institute of Biological Sciences
CANADA
e-mail; lilith.barry@nrc.ca

 ----------
From: Karen D. Larison
To: HistoNet@Pathology.swmed.edu
Subject: that sinking feeling
Date: Thursday, January 07, 1999 2:38PM

Hello histonetters,

I have a graduate student who has done some physiology on a monkey brain.
He
now wants to cut cryosections of this brain (electrode tracks have been
marked
with DiI), and has put a portion of the brain (maybe a 3-4" cube) in 30%
sucrose and is waiting patiently for it to sink.  He's been waiting for over
a
week now.  My question is:  Do all brains sink in 30% sucrose, or are the
brains of "higher" organism too myelin-rich to sink?  Any responses will be
appreciated.  He defends in about 3 weeks, and is a bit nervous as this work
represents his last piece of supporting data.

Thanks for all your help.

Karen Larison - University of Oregon