Tissue Shrinkage

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From:garygill <garygill@dcla.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Keep in mind that tissue shrinkage per se is not a bad thing.  Shrinkage is
part of the cross-linking process among proteins that hardens tissue and
makes it sectionable.  Note that different fixatives shrink tissues
different amounts so one should not use different fixatives without
anticipating such differences, among other possible changes.  It is
especially worth noting that any computer-based quantitative measurements of
cell and tissue features should be linked to the specific details of the
tissue and fixation/staining/ clearing process.  In other words, such
measurements are artifact dependent, and not measurements of fundamental
biologic features.

Baker's little book, as well as his larger one, are indeed classics.  It's
too bad they are out-of-print.

Gary Gill

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