Re: Tissue Shrinkage

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From:oshel@terracom.net (Philip Oshel) (by way of histonet)
To:histonet <histonet@magicnet.net>
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Ummm...no. Brain may also have these tables, but the one I thinking of was
in an electron microscopy volume of some kind (#&$(#&$#*QW with much
brow-beating). I haven't been able to find it.

M.A. Hayat does gives some % shrinkage data in his book " Introduction to
Biological Scanning Electron Microscopy", but his numbers are lower than
some that I've measured. He gives 10-43%, CNS being 10-15%, lymphocytes up
to 43% (diameter reduced from 8.3 to 4.8 microns). He also references the
work I *may* be remembering:
Boyde, A., E. Bailey, S.J. Jones, and A. Tamarin. 1977. Dimensional changes
during specimen preparation for scanning electron microscopy. In: O. Jahari
(ed.), Proceedings of the Tenth Annual SEM  Symposium, p 507. IITRI,
Chicago.
or perhaps:
Gusnard, D. and  R.H. Kirschner. 1977. Cell and organelle shrinkage during
preparation for scanning electron microscopy. J. Microscopy 110:51

Phil

>Philip (Oshel) referred to shrinkage tables.  I do not have it in
>front of me, but the table(s) may well have been in Brain's book -
>Preparation of Decalcified Sections,
>Russ Allison, Wales

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Philip Oshel
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oshel@terracom.net




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