silane or plus charge slides

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From:Gayle Callis <> (by way of histonet)
To:histonet <>
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Interesting comment about deterioration of plus charge slides.

I just talked to my Erie Scientific rep, and he said the opposite about
silanized slides, that they get better with age, but gave no scientific
basis for that statement, any comments?  I have always dated
poly l lysine slides, since this is a protein, and tried to use them
within 6 months.  I have also used plus charge (silanized variety)
after two years or so with the Microprobe syste they wostill work fine.

However, the newer lot seems to be the problem.  Thanks to the manufacturers
who put those invaluable lot numbers on products, at least they can see
if others are cplaining/having problems.

Beginning to think gremlins live in buffers.

Gayle Callis

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