<< Previous Message | Next Message >>
From:Frank Walmsley <frankw@U.Arizona.EDU> (by way of histonet)
To:histonet <histonet@magicnet.net>
Reply-To:
Content-Type:text/plain; charset="us-ascii"

Dear histonetters,
     I will be receiving some mouse skeletal muscle that has been injected
with flourescently labelled DNA.  It has been frozen in OCT and stored at
-80 amd will be frozen sectioned.  My question is what would be the best
post-treatment of the frozen sections in terms of fixation and
coverslipping for either confocal or UV microscopy. Would post fixation in
Acetone work or would this affect the flourochrome? Any suggestions would
be much appreciated!

						Frank Walmsley




.....................................................................
: Frank Walmsley, B.S.          Dept. of Cell Biology & Anatomy     :
: Research Specialist           University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)	        (email:  frankw@u.arizona.edu)      :
:...................................................................:
          http://www.cba.arizona.edu/histology-lab.html




<< Previous Message | Next Message >>