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From:Gayle Callis <> (by way of histonet)
To:histonet <>
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For undecalcifed bone, murine, you will need to use tungsten carbide
knives (Delaware Diamond Knives makes excellent quality, good pricing)

We use a Cryocut 1800, now an 1850 newer model number from Leica.

For undecalcified bone, you can do sections with their antiroll device
but using tape transfer system, Instrumedics, has greatly improved
our sectioning ability without tears/bad language!  AND I am able to remove
the Instrumedics device from the cabinet, whereas some cryostat
require permanent installment of the UV light/slide platform.  I
enjoy freedom to do whatever sectioning I desire, and we do hundreds of
frozens, mainly with disposables on soft tissues.

We use disposable knives, either high or low profile, and have the
other knife holder for steel c profile, or D profile tungsten carbide blades.

Their new disposable knife holder is wonderful, giving you full access to 100%
of the knife edge.  The 1850 is easy to clean, maintain, and a trouble
free machine.  I wish I had two!

Gayle Callis

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