Re: Questions about fixation and incubation
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From: | Jamie Erickson <JErickson@genetics.com> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi Donald,
I'm very interested in your work. I'm trying to stain w/ a
human antibody (IFN-g) in monkey skin biopsies treated with a
inflammatory agent. I use Acetone fixed frozen sections then post fix with
formalin prior to staining. I'm also trying to get CD4/8 to work in theses
samples that are paraffin embedded. The protocol I use from R&D systems and
It incubates overnight at room temperature. If you got this to stain w/ DAB
or Fitc I'd like to know exactly what you did. Please respond, you can also
reach me by phone at 978-623-1348.
Jamie Erickson
Genetics Institute
Andover, MA
>>> <RNAhybrid@aol.com> 01/23 1:38 PM >>>
I am a dermatologist at Mt Sinai Med Ctr in NYC. I am workingon a project
where i stained for cytokines in human skin, IL-4, IL-5, IFN gamma using
formalin fixation, overnight incubation at room temp and it worked ok. We are
also staining for CD-4, CD-8, CD-30, CD-1a and Factor XIIIa. Path recommended
we use acetone fixation and incubation overnight at 4 degrees cent. and
results have not been so good. I am not sure which fixation to use and
whether to incubate at room temp or 4 deg. Any recommendations will be
greatly appreciated.
Donald Rudikoff
!
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