Re: ER/PR tissues washing off

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From:Technical Services <> (by way of histonet)
To:histonet <>
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Hi Paula.

Our histology department prefers silanized slides over poly-L-lysine and
charged slides, especially for breast tissue that needs to undergo HIER.
Hope this helps.

Joel Weisenberger
DAKO Corporation
Technical Services
800-235-5743 x5325
-----Original Message-----
From: Paula Wilder <>
To: <>
Date: Monday, January 18, 1999 6:22 PM
Subject: IHC: ER/PR tissues washing off

>Hello Histonet!
>Been out of contact with the net for several months, so I hope this
>query is not a recent duplicate.  For some reason, all of a sudden, our
>ER and PR tissues have been washing off the slide after antigen
>retrieval using a rice steamer.  We use charged slides.  We have tried
>keeping the slides overnight in our incubator oven at 60 degrees.  We
>have thought about incubating the slides in a 75-80 degree oven for a
>shorter period of time, but have not done so yet.
>We have also thought about trying poly-L-lysine slides.  Do you have any
>thoughts?  Any information will be very greatly appreciated.  THANK
>Paula Wilder
>St. Joseph Medical Center
>Towson, MD
>Get Your Private, Free Email at

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