Re:CTS etc.
<< Previous Message | Next Message >>
| From: | Louise Burrell <lburrell@pathbox.wustl.edu> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Dear all---I have had CTR surgery on both hands; Also, the cortisone
injections into my elbows and wrists----they help for awhile then-ughhhhhh
I have found something wonderful---Glucosamine Chondroitin--you need 100
mg of glucosamine everyday for two weeks---then lower dosage. I am not
sure what the amount of chondroitin is---it is at every Walgreen's. The
best one is a little pink tablet. Works better than everything I have
tried. However I will get those bracelets, also...thanks
Louise
On Wed, 20 Jan 1999, HistoNet Server wrote:
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 01:33:19 -0600
> From: northstar44@juno.com (Mary L North)
> Subject: Mounting media
>
> To Cindi to whom I e-mailed info on IF mounting media and then realized I
> had designated Permount when I meant to write Permafluor. I deleted the
> message so I don't have individual address anymore. Sorry for the mixup.
> Mary North
>
> ___________________________________________________________________
> You don't need to buy Internet access to use free Internet e-mail.
> Get completely free e-mail from Juno at http://www.juno.com/getjuno.html
> or call Juno at (800) 654-JUNO [654-5866]
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 02:46:08 -0600
> From: LMGephart@aol.com
> Subject: Re: Lost sections
>
> Another opinion from the peanut gallery:
>
> We do use poly-l-lysine coated slides. We pick our sections off of the
> everyday waterbath that has gelatin added. We dry our sections at about 75
> degrees for 15 minutes. We use the routine stainer to deparaffinize and
> hydrate. It seems that we do everything that the elite say will not
>work, yet
> our tissue stays on and our stains look great.
>
> The only time we have a wash-off problem is when the tissue has not been
>fixed
> or processed correctly.
>
> - -Linda
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 03:30:13 -0600
> From: DORIT ZHARHARY <d_zharhary@sigma.co.il>
> Subject: RE: ER/PR tissues washing off
>
> Paula,
>
> I wonder whether your problems may stem from the slides themselves. Try the
> Poly-Prep slides or even better the Silane-Prep slides from Sigma. Both are
> coated to promote tissue section adherence.
>
> Dorit
>
> - ----------
> From: Paula Wilder
> Sent: eai uieue 19 edaao 1999 04:00
> To: HistoNet@Pathology.swmed.edu
> Subject: IHC: ER/PR tissues washing off
>
> Hello Histonet!
>
> Been out of contact with the net for several months, so I hope this
> query is not a recent duplicate. For some reason, all of a sudden, our
> ER and PR tissues have been washing off the slide after antigen
> retrieval using a rice steamer. We use charged slides. We have tried
> keeping the slides overnight in our incubator oven at 60 degrees. We
> have thought about incubating the slides in a 75-80 degree oven for a
> shorter period of time, but have not done so yet.
> We have also thought about trying poly-L-lysine slides. Do you have any
> thoughts? Any information will be very greatly appreciated. THANK
> YOU!!!!
>
>
> Paula Wilder
> St. Joseph Medical Center
> Towson, MD
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 07:30:30 -0600
> From: Mike Bromley <MBromley@PICR.man.ac.uk>
> Subject: antibodies
>
> Dear all
>
> Does anyone have any experience of using commercial companies to
> obtain polyclonal antibodies to novel proteins? What we would like to
> do, is to run the partially purified proteins on SDS PAGE, cut bands
> out of the gels and then send them off to be injected into a suitable
> animal (eg rabbit). Has anyone tried such an approach and does anyone
> know of a good company that we could use?
>
> In a similar vein, we have also heard that there are companies that
> will do protein sequencing again from bands on gels. Does anyone have
> any experience of this?
>
> Many thanks
>
> Mike
>
> Michael Bromley PhD
> Histology Department
> Paterson Institute
> Wilmslow Road
> Manchester M20 9BX
> UK
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 07:40:21 -0600
> From: "Pete de Forest" <fpd24093@hotmail.com>
> Subject: Human Pancreas Cells - Tissue Culture
>
> Can someone site any good references (lab protocol type articles) on
> tissue culture of human pancreas cells. I am a beginner in this field,
> so any good advice would be greatly appreciated.
>
> Peter de Forest
> University of Munich, Germany
> School of Medicine
>
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 07:50:54 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: Mounting media
>
> Cindi,
> I use Dako Faramount for my AEC immunos. don't know if it will work
> with flourescence. Have tried several others an this is what I like best.
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
>
> > ----------
> > From: Cindipqr@aol.com[SMTP:Cindipqr@aol.com]
> > Sent: Monday, January 18, 1999 9:25 AM
> > To: histonet@Pathology.swmed.edu
> > Subject: Mounting media
> >
> > Hello Everyone,
> > I need some recommendations for a mounting media. It needs to be
> > water
> > soluble for 100 um. sections of immunofluorescence. The media I'm using
> > now
> > doesn't always dry so the coverslips are moving. This is quite a problem
> > for
> > confocal microscopy.
> > Thanks in advance,
> > Cindi
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 08:02:08 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: lens histology
>
> Judy,
> I took a vet histology course at NSH in NM and this was covered.
> Still have my notes and will dig them out if necessary. Let me know.
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
>
> > ----------
> > From: Judy Trogadis[SMTP:judy@playfair.utoronto.ca]
> > Sent: Friday, January 15, 1999 12:40 PM
> > To: HistoNet@Pathology.swmed.edu
> > Subject: lens histology
> >
> > Dear Histoexperts:
> >
> > We are planning to do immunostaining on human eye lens tissue. Is there
> > any
> > special fixation protocol that would prevent the lens from becoming too
> > brittle and difficult to section? I have also heard that the sections can
> >
> > detach from slides easily. Is any substrate optimal to prevent this?
> > I can already tell that the technical components of this project will be
> > tough to work out, however, from past experience, I also know that this
> > group has the answer to everything.
> >
> > Hopefully,
> > judy
> >
> >
> > Judy Trogadis
> > Eye Research Institute and
> > University of Toronto
> > Toronto Hospital, Western Div.
> > 399 Bathurst St.
> > Toronto, Canada M5T 2S8
> >
> > phone: 416-603-5088
> > Fax: 416-603-5126
> > email: judy@playfair.utoronto.ca
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 08:02:32 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: tissue for htl practical exam
>
> Katrina,
> Thought NSH had a bank.
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
>
> > ----------
> > From: Katrina Knott[SMTP:knottk@amc.org]
> > Sent: Friday, January 15, 1999 3:47 PM
> > To: histonet@pathology.swmed.edu
> > Subject: tissue for htl practical exam
> >
> > Hey all,
> > I am looking for some tissue for my histotechnologist practical
> > coming up
> > in April. I was very lucky to receive several tissues already, but am
> > still
> > searching for a few more. Does anyone know how I can get human pancreas
> > and
> > tissue containing Heliobactor pylori? Any help is greatly appreciated.
> >
> > Thanks,
> > Katrina
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 08:26:43 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: Antibody Lot Test Question
>
> Sandosis,
> My understanding of lot numbers is that they are batch numbers.
> Large volume that has been aliquot. Therefore I would not mix different lot
> numbers. I would also not mix the different samples from the same lot. Each
> aliquot has the potential of being subjected to detrimental treatment,
> temperature would be an example. I would check a new aliquot of antibody
> even with the same lot number for the same reason.
> I view lot numbers as a way of checking with the company if I
> happens to have problems at the bench.
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
>
>
> > ----------
> > From: sjrugby@juno.com[SMTP:sjrugby@juno.com]
> > Sent: Friday, January 15, 1999 9:02 PM
> > To: histonet@pathology.swmed.edu
> > Subject: Antibody Lot Test Question
> >
> > Histonetters,
> > A couple of immnu lot testing queries....
> >
> > -How do you feel about mixing primary antibody lots together when there
> > isn't enough of the old lot.
> > Whether you say "Yay" or "Nay", please include your reasoning.
> >
> > -If a new antibody lot arrives in the lab and it has the same lot number
> > as the current tested antibody, is it necessary to test it? Again,
> > please share your reasons for agreeing or disagreeing.
> >
> > -What DO you do with a drunken sailor?
> >
> > Many thanks,
> > Sandosis
> >
> > ___________________________________________________________________
> > You don't need to buy Internet access to use free Internet e-mail.
> > Get completely free e-mail from Juno at http://www.juno.com/getjuno.html
> > or call Juno at (800) 654-JUNO [654-5866]
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 08:40:26 -0600
> From: "Margaret Gondo" <gondom@genemedicine.com>
> Subject: Re: IHC: ER/PR tissues washing off
>
> Paula-
>
> I don't think this is your problem (especially if you are using
> commercially made retrieval solutions), but whenever I've used basic
> retrieval solutions (ie EDTA pH10) for antigen retrieval my sections tend
> to come off a bit.
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 09:15:32 -0600
> From: mark.lewis@shandon.com
> Subject: RE: Mounting media
>
> Permafuor can be obtained from Shandon
>
> ----------
> From: northstar44@juno.com [SMTP:PC :northstar44@juno.com]
> Sent: Tuesday, January 19, 1999 2:47 AM
> To: histonet@pathology.swmed.edu
> Subject: Mounting media
>
> <<File: ENVELOPE.TXT>>
> --------------------------------------------------------------------------
> --
> To Cindi to whom I e-mailed info on IF mounting media and then realized I
> had designated Permount when I meant to write Permafluor. I deleted the
> message so I don't have individual address anymore. Sorry for the mixup.
> Mary North
>
>
> ___________________________________________________________________
> You don't need to buy Internet access to use free Internet e-mail.
> Get completely free e-mail from Juno at http://www.juno.com/getjuno.html
> or call Juno at (800) 654-JUNO [654-5866]
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 09:16:04 -0600
> From: sbledsoe <sbledsoe@iupui.edu>
> Subject: Lung Embedding Protocol
>
> I have been asked to Post the following:
>
> Could someone please suggest a good protocol for embedding lung tissue in
> paraffin.
>
>
>
> Thanks
>
> Sharon B. Bledsoe
> Indiana University
> School of Medicine
> Dept. of Surgery
> Indianapolis, IN 46202
> sbledsoe@iupui.ed
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 09:46:35 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: sections coming off for ER/PR
>
> I am having similar problems with frozen sections, bubbling up, looking
> strung out across the slide. Could it be a bad lot of plus charged
> slides? When something like this suddenly happens, and nothing else has
> changed in the remainder of the procedure, I get antsy about the slides.
> And I do not require any antigen retrieval.
>
> Have changed fixative to fresh, new buffers, etc ad nauseum, and still
> have problems. My usual double acetone fixation protocol, plus long
> air drying before fix (longer now!) hasn't improved the problem.
>
> Has anyone else run into bad lots of plus charged slides? They are usually
> the one thing that is truly reliable, but maybe not in this case.
>
> Gayle Callis
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 09:47:33 -0600
> From: "Harclerode, Donna" <DHarclerode@ligand.com>
> Subject: Androgen receptor in rat tissue
>
> Richard,
> Novacastra (available from Vector in the US) has a polyclonal (catalog
> NCL-ARp) that works on rat paraffin sections (on hamster too). I use it
> at 1:40 overnight 4oC incubation with microwave citrate buffer
> pretreatment. I use a labeled strep avidin -HRP system with DAB. There
> is more background than I would prefer but the monoclonals I tried have
> not worked.
>
> Donna Harclerode HT (ASCP) HTL, QIHC
> Ligand Pharmaceuticals
> San Diego, CA
>
>
> [Harclerode, Donna] Date: 18 Jan 1999 09:15:34 -0600
> From: "Richard Edwards" <REE3@leicester.ac.uk>
> Subject: Antibody search
>
>
>
> HELP PLEASE
>
> Anyone out there actually using an anti-androgen receptor antibody
> that
> works on paraffin sections of rat tissue??????
>
> Cheers
> Richard Edwards
> MRC
> TOXICOLOGY
> !
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 10:15:27 -0600
> From: rkline@emindustries.com
> Subject: Re: Lung Embedding Protocol
>
> Sharon,
>
> I'm not sure I understand your question. Are you having problems with the
> processing you are doing now? If you are what problems are you having?
> Are you just starting to process lung?
>
> I always processed lung tissue the same as routine. Fixation in 10% NBF.
> Just make sure it is fixed thoroughly. This included ressections and
> biopsy. Also, avoid large and thick tissue sections. Residents and some
> pathologists have the tendency to think larger is better.
>
> Rande Kline HT (ASCP)
> Technical Services
> EM Science
>
>
>
>
> sbledsoe <sbledsoe@iupui.edu> on 01/19/99 11:10:57 AM
>
> To: HistoNet@Pathology.swmed.edu
> cc:
> Subject: Lung Embedding Protocol
>
>
>
>
> I have been asked to Post the following:
>
> Could someone please suggest a good protocol for embedding lung tissue in
> paraffin.
>
>
>
> Thanks
>
> Sharon B. Bledsoe
> Indiana University
> School of Medicine
> Dept. of Surgery
> Indianapolis, IN 46202
> sbledsoe@iupui.ed
>
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 10:15:57 -0600
> From: rkline@emindustries.com
> Subject: RE: Antibody Lot/More About Lot Numbers
>
> Cynthia,
>
> Thanks for the good information. I wanted to touch on lot numbers a little
> further.
>
> Lot numbers are given to batches. Batch numbers trace back to raw
> materials and may trace back to other information such as date of
> manufacture and location of manufacturing. The information that a lot
> number contains is dependent on the manufacturer. In the case of
> antibodies, I would agree with not mixing lots. The manufacturing
> practices, specifications and applications are too delicate.
>
> The lot number is important information to give to the manufacturer along
> with a problem. ISO and GMP facilities have complaint systems in place.
> Also, they do tracking of complaints and have to show resolution to
> problems. It's an opportunity for improvement on the manufacturers end and
> an opporutnity for end-users to get the products they need to work with.
> Manufacturers need to hear complaints (along with the good stuff).
>
> Regards,
> Rande Kline HT (ASCP)
> Technical Services
> EM Science
>
>
>
>
>
>
>
>
> Cynthia Favara <cfavara@atlas.niaid.nih.gov> on 01/19/99 09:17:00 AM
>
> To: "histonet@pathology.swmed.edu" <HistoNet@Pathology.swmed.edu>,
> "'sjrugby@juno.com'" <sjrugby@juno.com>
> cc:
> Subject: RE: Antibody Lot Test Question
>
>
>
>
> Sandosis,
> My understanding of lot numbers is that they are batch numbers.
> Large volume that has been aliquot. Therefore I would not mix different lot
> numbers. I would also not mix the different samples from the same lot. Each
> aliquot has the potential of being subjected to detrimental treatment,
> temperature would be an example. I would check a new aliquot of antibody
> even with the same lot number for the same reason.
> I view lot numbers as a way of checking with the company if I
> happens to have problems at the bench.
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
>
>
> > ----------
> > From: sjrugby@juno.com[SMTP:sjrugby@juno.com]
> > Sent: Friday, January 15, 1999 9:02 PM
> > To: histonet@pathology.swmed.edu
> > Subject: Antibody Lot Test Question
> >
> > Histonetters,
> > A couple of immnu lot testing queries....
> >
> > -How do you feel about mixing primary antibody lots together when there
> > isn't enough of the old lot.
> > Whether you say "Yay" or "Nay", please include your reasoning.
> >
> > -If a new antibody lot arrives in the lab and it has the same lot number
> > as the current tested antibody, is it necessary to test it? Again,
> > please share your reasons for agreeing or disagreeing.
> >
> > -What DO you do with a drunken sailor?
> >
> > Many thanks,
> > Sandosis
> >
> > ___________________________________________________________________
> > You don't need to buy Internet access to use free Internet e-mail.
> > Get completely free e-mail from Juno at http://www.juno.com/getjuno.html
> > or call Juno at (800) 654-JUNO [654-5866]
> >
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 10:43:47 -0600
> From: "Technical Services" <techserv@dakousa.com>
> Subject: Re: ER/PR tissues washing off
>
> Hi Paula.
>
> Our histology department prefers silanized slides over poly-L-lysine and
> charged slides, especially for breast tissue that needs to undergo HIER.
> Hope this helps.
>
> Joel Weisenberger
> DAKO Corporation
> Technical Services
> 800-235-5743 x5325
> techserv@dakousa.com
> - -----Original Message-----
> From: Paula Wilder <histo20@hotmail.com>
> To: HistoNet@Pathology.swmed.edu <HistoNet@Pathology.swmed.edu>
> Date: Monday, January 18, 1999 6:22 PM
> Subject: IHC: ER/PR tissues washing off
>
>
> >Hello Histonet!
> >
> >Been out of contact with the net for several months, so I hope this
> >query is not a recent duplicate. For some reason, all of a sudden, our
> >ER and PR tissues have been washing off the slide after antigen
> >retrieval using a rice steamer. We use charged slides. We have tried
> >keeping the slides overnight in our incubator oven at 60 degrees. We
> >have thought about incubating the slides in a 75-80 degree oven for a
> >shorter period of time, but have not done so yet.
> >We have also thought about trying poly-L-lysine slides. Do you have any
> >thoughts? Any information will be very greatly appreciated. THANK
> >YOU!!!!
> >
> >
> >Paula Wilder
> >St. Joseph Medical Center
> >Towson, MD
> >
> >______________________________________________________
> >Get Your Private, Free Email at http://www.hotmail.com
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 10:44:32 -0600
> From: "Berger, Jennifer" <jberger@lrri.org>
> Subject: paraplast removal
>
> I know that this has been discussed before, but we had a technician spill
> hot paraplast on her skirt. What is the best way to remove it?
> Thanks
> Jennifer Berger
> Lovelace Respiratory Research Institute
> Albuquerque, NM
> jberger@lrri.org
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 10:45:11 -0600
> From: VICKI_GAUCH@CCGATEWAY.AMC.EDU
> Subject: Re: Microtomes and Carpel Tunnel Syndrome
>
> Christine,
> We have several techs in our laboratory suffering from
> wrist,shoulder and elbow pain diagnosed as repetitive motion injuries.
> I personally have lateral epicondylitis of the left elbow from
> cutting and have had the cortisone shots, physical therapy,etc. but
> the only thing that has given any consistent relief is a magnetic band
> I wear when cutting and at night. Believe it or not,it does work. If
> you get any information on any of the RMI's would you please forward
> it to me ? Thank you in advance for your help...
>
> Vicki
> Albany Medical Center
> ______________________________ Reply Separator
> _________________________________
> Subject: Microtomes and Carpel Tunnel Syndrome
> Author: christine lee <C.lee@uq.net.au> at Internet-Mail
> Date: 1/15/99 10:28 PM
>
>
> A few years ago I was aware of debate in histology laboratories regarding
> the repeditive stress injuries caused by continued use of microtomes, but
> didn't keep any of the relevant literature. Can anyone refere me to relevant
> literature or websites, or is there anyone who has suffered any repeditive
> strain injuries.
> I would love to hear from you so I can convince our pathologists that it
> does exist. Thanks.
>
> Christine Lee
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 11:23:57 -0600
> From: "M.A. Jennings" <jennings@mayo.edu>
> Subject: Re: antibodies/Bromley
>
> Dear Michael
> I guess I responded to your inquiry mostly to let you know that it is not
> so far fetched of an idea and because it brought back some old memories.
> You may want to try some of your local universities. The University of
> South Carolina had an antibody production laboratory that did just what you
> asking. We did 2-D gels and cut the spots out of UNSTAINED gels. That was
> "fun". We stockpiled quite a bit before we actually started injections. (12
> gels/day 4 days/week for a month). If you would like me to contact them
> and see if they take outside work or get you the procedures with references
> I would be glad to. Now the best way, (a LOT less work) and I believe that
> is where you are going with your next question, is to get the sequence,
> have peptides sequenced and then attached to glass beads for injection and
> antibody production. There are quite a few companies, I believe "Genetics
> Sequencing" is one, that sequence proteins from gel bands. I'll get you
> some more specifics (names phone # etc.) if you don't get responses from
> them directly to this inquiry. good luck anita
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 11:36:22 -0600
> From: sknauer@nexstar.com
> Subject: gross photography setup
>
> Does anyone have a really good gross photography set-up that would be
> willing to share the specs? We need to put a system together for 35mm film
> and appropriate lighting.
> Susan Knauer
> sknauer@nexstar.com
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 11:37:32 -0600
> From: "Hendry, Chris I" <HendryC@mar.dfo-mpo.gc.ca>
> Subject: RE: paraplast removal
>
> Household iron and paper towel has worked for me. Simply put the paper
> towel over the hardened paraplast, iron over the towel, and the melted
> paraffin absorbs into the paper towel. Good luck.
>
> Chris Hendry
> Department of Fisheries and Oceans
> Biological Station
> St. Andrews, NB E0G 2X0 Canada
> (506) 529-8854 Phone
> (506) 529-5862 Fax
> e-mail: hendryc@mar.dfo-mpo.gc.ca
> URL: http://www.geocities.com/CapeCanaveral/Hall/9440
>
> To steal ideas from one person is plagiarism; to steal from many is
> research.
>
> > -----Original Message-----
> > From: Berger, Jennifer [SMTP:jberger@lrri.org]
> > Sent: Tuesday, January 19, 1999 12:34 PM
> > To: 'histonet'
> > Subject: paraplast removal
> >
> > I know that this has been discussed before, but we had a technician spill
> > hot paraplast on her skirt. What is the best way to remove it?
> > Thanks
> > Jennifer Berger
> > Lovelace Respiratory Research Institute
> > Albuquerque, NM
> > jberger@lrri.org
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 12:01:57 -0600
> From: "Weems, Joyce" <JWEEMS@sjha.org>
> Subject: RE: paraplast removal
>
> Freeze it and peel as much off as possible. Then place it paraffin side
> down on brown paper bags over a towel. The paper bags should absorb the
> paraffin! J
>
> >----------
> >From: Berger, Jennifer[SMTP:jberger@lrri.org]
> >Sent: Tuesday, January 19, 1999 11:33 AM
> >To: 'histonet'
> >Subject: paraplast removal
> >
> >I know that this has been discussed before, but we had a technician spill
> >hot paraplast on her skirt. What is the best way to remove it?
> >Thanks
> >Jennifer Berger
> >Lovelace Respiratory Research Institute
> >Albuquerque, NM
> >jberger@lrri.org
> >
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 12:02:31 -0600
> From: Elizabeth Morehead <DREWSB@smtpgw2.musc.edu>
> Subject: Lab Comparisons
>
> Thanks to all who responded to the question about number of techs vs. number
> of accessions
> in large facilities. I've compiled the submitted data, but still waiting on
> some of ya'll (you know
> who you are!)
> Please respond and you'll get the completed list!
> Thanks-
> Beth Morehead
> Med U of SC
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 12:26:12 -0600
> From: larisonk@UONEURO.uoregon.edu
> Subject: Forwarded: Anti-Hu MAb 16A11
>
> Histonetters,
>
> Someone on the net recently suggested that anti-hu antibodies aren't
> available.
> This isn't true. The Monoclonal Facility here at the University of
>Oregon has
>
> made an anti-hu monoclonal. Mike Marusich, who directs the monoclonal
> facility, wanted me to post the following note on the availability and
> characteristics of this anti-hu antibody.
>
> Karen Larison - University of Oregon
>
> ***********************************************************************
>
> Anti-Hu MAb 16A11 is a robust early marker of vertebrate
> neurogenesis. The antigen(s) detected by MAb 16A11 are expressed
> concomitant with or shortly after neuronal terminal mitosis, and are
> expressed at high levels in neuron cell bodies. The exclusive cell
> body expression of neuronal Hu proteins makes Anti-Hu MAb 16A11
> extremely useful for enumeration of early neurons (unlike
> neurofilament and neurofilament associated proteins).
>
> MAb 16A11 is a mouse IgG2b that binds specifically to a conserved
> peptide epitope present in most known members of the Hu family of
> vertebrate neuronal proteins. Thus, MAb 16A11 binds to human proteins
> HuD and HuDpro (alternatively spliced forms of the HuD gene product)
> as well as HuC (the HuC gene product). In addition, the epitope
> recognized by MAb 16A11 is present in the predicted amino acid
> sequences of HuCLS (an alternatively spliced form of the HuC gene
> product), and Hel-N1 (the gene product of the third member of the
> mammalian Hu gene family). The only known Hu protein isoform that
> does not contain the amino acid domain recognized by MAb 16A11
> is HuDmex, which is a minor constituent of the Hu proteins present in
> human brain. Western blot immunoassays of human, mouse, avian
> and fish neural tissue extracts, and recombinant human HuD and HuC
> proteins were used to document the specificity of antibody binding to
> Hu proteins. Particle Concentration Fluorescence Immunoassays of
> synthetic peptides were used to identify the 16A11 epitope.
> Immunohistochemical analyses of human, mouse, avian and fish
> tissues documented the tissue and developmental patterns of Hu
> protein-immunoreactivity.
>
> Reference:
>
> Marusich, M. F., Furneaux, H. M., Henion, P., Weston, J. A. (1994).
> Hu neuronal proteins are expressed in proliferating neurogenic cells.
> J. Neurobiol. 25, 143-155.
>
> To facilitate distribution of this reagent, an initial sample of MAb
> 16A11 will be provided at no charge to any investigator who requests
> it. However, additional samples require payment of $120 per 275 ug
> sample to recover production and distribution costs.
>
> For more information contact:
>
> Michael Marusich, Ph.D.
> Director Monoclonal Antibody Facility
> Institute of Neuroscience
> University of Oregon
> Eugene, OR 97403-1254
>
> ph: 541-346-5688
> fax: 541-346-4548
> email: marusich@uoneuro.uoregon.edu
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 12:26:37 -0600
> From: "Tim Morken" <timcdc@hotmail.com>
> Subject: Re: gross photography setup
>
> The absolute best gross photo set-up is made by Photodyne of Califonia
> (http://www.photodyne.com/). I have used this and would never buy
> anything else, given the choice. Best of all, you can do 35 mm
> photography or digital imaging without modifying the setup.
>
> Tim Morken, B.S., EMT(MSA), HTL(ASCP)
> Infectious Disease Pathology
> Centers for Disease Control
> MS-G32
> 1600 Clifton Rd.
> Atlanta, GA 30333
> USA
>
> email: tim9@cdc.gov
> timcdc@hotmail.com
>
> FAX: (404)639-3043
>
>
> - ----Original Message Follows----
> Date: Tue, 19 Jan 1999 09:49:23 -0700
> From: sknauer@nexstar.com
> Subject: gross photography setup
> To: HistoNet@pathology.swmed.edu
>
> Does anyone have a really good gross photography set-up that would be
> willing to share the specs? We need to put a system together for 35mm
> film
> and appropriate lighting.
> Susan Knauer
> sknauer@nexstar.com
>
>
>
>
>
>
>
>
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 12:27:12 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: paraffin removal
>
> The hot iron/paper towl works well, and if you can get a brown
> paper towel, you will see the melted paraffin, keep changing the towel
> until you see no more melted paraffin, then a dry cleaning will finalize
> the residual removal. I have even (if you test an area for color) used
> a bit of xylene substitute (not the orange juice smelling stuff) dabbed
> it on and blotted it out for final step. Use a hood, gloves for that.
>
> This is an old method for removing candle wax from favorite table cloths,
> and if you ice the paraffin droplets first, gently lever off, and then
> use the iron, you will not melt more wax into the fabric.
>
> Good luck
> Gayle Callis
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 13:04:05 -0600
> From: "Katrina Knott" <knottk@amc.org>
> Subject: books for histology
>
> Kimberly in Wyoming
> I have been studying from the Histotechnology, A Self-instructional Text
> written by Freida Carson. It also comes with a study guide. I have the
> 1990 edition and it seems to be working well. I know that she has written
> another edition of this book this year and it can be ordered through the
> Board of Registry
> Along with that I have my supervisor's old histology text to look up
> different cells and what they do. I use it along with Carson's book for a
> reference. It helps me to understand better what each stain is for and how
> it works. Any histology text should do. It is always nice to have colored
> pictures. I think that the BOR will probably have something with that too.
> I am using the Textbook of Histology by Leeson, Leeson and Paporro, 1985.
> I also recently purchased the BOR study guide, Histolotechnology
> Examinations. It has been very helpful. It is divided into several
> sections and asks many many questions. It helps to practice how the
> question will be asked and is in a multiple choice format. It also came
> with a computer disk that help you practice computerized tests.
> I hear that Sheehan wrote a book that is also really good to study
>from. I
> am in the process of finding it.
> The Bor web page is helps a lot. http://www.ascp.org/index.asp They
>have a
> lot of books that you can choose from. And you can purchase most of them
> from there, or you can email them for their suggestions.
>
> I hope that this will help you and Good Luck!
> Katrina
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 13:31:04 -0600
> From: George McNamara <geomcnamara@earthlink.net>
> Subject: Re: Daily Digest
>
> Dear Cindi,
>
> Try VectaShield(R) from
>
> Vector Laboratories
> 30 Ingold Road
> Burlingame, CA 94010
> To order, call: (800) 227-6666
>
> For technical assistance:
> TEL: (650) 697-3600
> FAX: (650) 697-0339
> E-mail: vector@vectorlabs.com
>
>
> http://www.vectorlabs.com (Molecular Biology Apps ->
> Fluorescence/VectaShield).
>
> Incidentally, their recommended 25 ul for a 22x22 mm coverglass seems a
> bit excessive in terms of potential for excess liquid.
>
> For slippery overglasses, try wicking out excess fluid with a kimwipe.
>
>
> As for coverglasses, you might try sealing them with any one of:
>
> * vaseline (apply from syringe without needle, but don't get on objective
> lens!).
> * crazy glue pen (my favorite, let it dry a few minutes before putting on
> microscope).
> * VALAP (a 1:1:1 solution of vaseline: lanolin:paraffin that is heated to
> melt and then painted around the coverglass; make sure it's dry before
> putting on scope. I have no idea of the melting temperature or how to stir
> them together).
>
>
>
> >Date: 18 Jan 1999 10:31:17 -0600
> >From: Cindipqr@aol.com
> >Subject: Mounting media
> >
> >Hello Everyone,
> > I need some recommendations for a mounting media. It needs to be water
> >soluble for 100 um. sections of immunofluorescence. The media I'm using now
> >doesn't always dry so the coverslips are moving. This is quite a
>problem for
> >confocal microscopy.
> >Thanks in advance,
> >Cindi
>
>
>
> George McNamara, Ph.D.
> Applied Spectral Imaging, Inc.
> 2120 Las Palmas Drive, Suite D
> Carlsbad, CA 92009
> voice 760-929-2840 ext 17
> fax 760-929-2842
> geomcnamara@earthlink.net
> http://www.spectral-imaging.com (company)
> http://home.earthlink.net/~geomcnamara/Index.html (personal)
> Major bio-medical imaging applications:
> SKY - Spectral Karyotyping
> SPY - Spectral Pathology
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 14:16:15 -0600
> From: "Jeff Silverman" <peptolab@hamptons.com>
> Subject: Re: Lost sections ER/PR
>
> It has been my experience that Positive-charged slides can deteriorate with
> age and that they should have an expiration date. We pick up our sections
> from the regular water bath that has a tiny pinch of gelatin added and dry
> them overnight or even over the weekend in a 60 degree C oven. Of course,
> poorly processed tissue will wash off no matter what you try.
>
> Also, a repeat question- Anyone aware of where I can find antibody Ki M1p-
> a macrophage marker?
>
> Jeff Silverman
> Southampton Hospital NY USA
>
> - ----------
> > From: LMGephart@aol.com
> > To: bbracing@silk.net; histonet@Pathology.swmed.edu
> > Subject: Re: Lost sections
> > Date: Tuesday, January 19, 1999 3:38 AM
> >
> > Another opinion from the peanut gallery:
> >
> > We do use poly-l-lysine coated slides. We pick our sections off of the
> > everyday waterbath that has gelatin added. We dry our sections at about
> 75
> > degrees for 15 minutes. We use the routine stainer to deparaffinize and
> > hydrate. It seems that we do everything that the elite say will not
> work, yet
> > our tissue stays on and our stains look great.
> >
> > The only time we have a wash-off problem is when the tissue has not been
> fixed
> > or processed correctly.
> >
> > -Linda
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 14:29:28 -0600
> From: "Bourassa, Patricia" <patricia_bourassa@groton.pfizer.com>
> Subject: CD4 and CD8 IHC
>
> Hi, all!
>
> Has anyone stained for CD4 and CD8 in mouse tissue? I was wondering what
> conditions people have found to be effective. How are the levels in spleen?
>
> Thanks.
>
> - -Patti
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 14:43:53 -0600
> From: larisonk@UONEURO.uoregon.edu
> Subject: Re: Mounting media
>
> Cindi,
>
> You might try Mowiol from Calbiochem. You have to make it up yourself
>from a
> polyvinyl alcohol solid. They give you the protocol. It's supposed to
> prevent
> photobleaching, although I've found that this depends alot on the
>fluorophore
> used. It takes about a day to dry.
>
> Karen Larison - University of Oregon
>
>
> Date: Mon, 18 Jan 1999 11:25:46 -0500 (EST)
> From: Cindipqr@aol.com
> Subject: Mounting media
> To: histonet@Pathology.swmed.edu
>
> Hello Everyone,
> I need some recommendations for a mounting media. It needs to be water
> soluble for 100 um. sections of immunofluorescence. The media I'm using now
> doesn't always dry so the coverslips are moving. This is quite a problem for
> confocal microscopy.
> Thanks in advance,
> Cindi
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 14:56:59 -0600
> From: garygill <garygill@dcla.com>
> Subject: RE: Lost sections ER/PR
>
> The persistence of a problem despite many "solutions" is often an indication
> that the underlying fundamental cause has not been identified. In the case
> of tissue sections adhesion is promoted by mutually attractive surfaces
> (i.e., opposite electrical charges) in contact with one another. Tissue and
> glass are naturally attractive under the right conditions. Specifically,
> the glass must be squeaky clean and the tissue must lie flat against the
> glass. Folds can contract during fixation and help separate the sections.
>
> To make glass wettable, simply immerse slides in alcohol and wipe dry with
> clean cheesecloth immediately before use. Precleaned and/or coated glass
> will naturally adsorb electrical atmospheric/particulate charges over time
> and become less wettable (hence Jeff's remark that "Positive-charged slides
> can deteriorate with age"). Using this method in cytology results in good
> cell recovery and flattening. This method is better than frosted slides,
> albuminized slides, albuminized cell buttons, poly-L-lysine coated slides,
> etc. BTW, moisture that naturally condenses between packed slides over time
> interacts with the silica and forms HCl, which etches the glass and leaves
> the whitish deposit we see, furthering reducing attractive forces. Thus,
> Clay Adams began packaging its slides in hermetically sealed wrap with
> silica gel packed inside many years ago. Cover glass manufacturers followed
> suit and include small packets in the 1-oz plastic containers.
>
> Gary Gill
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 15:13:59 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: paraplast removal
>
> The skirt or the paraplast - sorry its been tht kind of day. A hot iron with
> tissue paper/paper towel underneath should work nicely. I would use it as
> the perfect excuse to get a new outfit.
> Cynthia Favara
>
> > ----------
> > From: Berger, Jennifer[SMTP:jberger@lrri.org]
> > Sent: Tuesday, January 19, 1999 9:33 AM
> > To: 'histonet'
> > Subject: paraplast removal
> >
> > I know that this has been discussed before, but we had a technician spill
> > hot paraplast on her skirt. What is the best way to remove it?
> > Thanks
> > Jennifer Berger
> > Lovelace Respiratory Research Institute
> > Albuquerque, NM
> > jberger@lrri.org
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 15:14:38 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: CD4 and CD8 IHC
>
> Patti,
> As I recall we tried this on frozen sectons - recall that it worked
> nicely with a directly HRP conjugated secondary. PI lost interest.
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
>
> > ----------
> > From: Bourassa, Patricia[SMTP:patricia_bourassa@groton.pfizer.com]
> > Sent: Tuesday, January 19, 1999 1:26 PM
> > To: Histonet @Pathology.Swmed.Edu (E-mail)
> > Subject: CD4 and CD8 IHC
> >
> > Hi, all!
> >
> > Has anyone stained for CD4 and CD8 in mouse tissue? I was wondering what
> > conditions people have found to be effective. How are the levels in
> > spleen?
> >
> > Thanks.
> >
> > -Patti
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 15:15:25 -0600
> From: Jamie Erickson <JErickson@genetics.com>
> Subject: Re: CD4 and CD8 IHC
>
> Patricia,
> I stain for CD4; CD8 routinely in mouse spleen a normal mouse
> spleen is loaded w/ both. So far only frozens work. Cryosection then acetone
> fixed for 1 minute then stored at -20 til stained. I use a techmate
> autostainer from ventanna. The antibodies are from Pharmingen CD4 cat
>#01071D,
> 1ug/ml--working Conc, CD8a 01041D, 5ug/ml--working Con. Very easy very nice
> staining.
>
> Jamie Erickson
> Genetics Institute
>
>
> >>> "Bourassa, Patricia" <patricia_bourassa@groton.pfizer.com> 01/19 3:26 PM
> >>>
> Hi, all!
>
> Has anyone stained for CD4 and CD8 in mouse tissue? I was wondering what
> conditions people have found to be effective. How are the levels in spleen?
>
> Thanks.
>
> - -Patti
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> !
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 17:15:30 -0600
> From: Frank Walmsley <frankw@U.Arizona.EDU>
> Subject:
>
> Dear histonetters,
> I will be receiving some mouse skeletal muscle that has been injected
> with flourescently labelled DNA. It has been frozen in OCT and stored at
> - -80 amd will be frozen sectioned. My question is what would be the best
> post-treatment of the frozen sections in terms of fixation and
> coverslipping for either confocal or UV microscopy. Would post fixation in
> Acetone work or would this affect the flourochrome? Any suggestions would
> be much appreciated!
>
> Frank Walmsley
>
>
>
>
> .....................................................................
> : Frank Walmsley, B.S. Dept. of Cell Biology & Anatomy :
> : Research Specialist University of Arizona :
> : (office: AHSC 4212) P.O. Box 245044 :
> : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
> : (FAX: 520-626-2097) (email: frankw@u.arizona.edu) :
> :...................................................................:
> http://www.cba.arizona.edu/histology-lab.html
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 17:15:51 -0600
> From: "Jeff Silverman" <peptolab@hamptons.com>
> Subject: Good waterbath
>
> Netters,
> Anyone know of a good, inexpensive table top waterbath suitable for heating
> 2 or 3 coplin jars to 95 degrees C for Her2neu antigen retrieval?
>
> Jeff Silverman
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 17:16:14 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: silane or plus charge slides
>
> Interesting comment about deterioration of plus charge slides.
>
> I just talked to my Erie Scientific rep, and he said the opposite about
> silanized slides, that they get better with age, but gave no scientific
> basis for that statement, any comments? I have always dated
> poly l lysine slides, since this is a protein, and tried to use them
> within 6 months. I have also used plus charge (silanized variety)
> after two years or so with the Microprobe syste they wostill work fine.
>
> However, the newer lot seems to be the problem. Thanks to the manufacturers
> who put those invaluable lot numbers on products, at least they can see
> if others are cplaining/having problems.
>
> Beginning to think gremlins live in buffers.
>
> Gayle Callis
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 18:00:52 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: murine CD4/8, reply
>
> I agree with Jamie Erickson. We use PharminGen antibodies with same
> concentrations, frozens (our fixation is a bit longer with acetone)
> but results are excellent. We prefer F(ab)'2 secondaries, mouse and
> human absrbed as secondary, but a new twist to using a secondary,
> and it does work! is to use a mouse antirat-IgG (H+L) F(ab)'2 biotinlyated
> secondary, 1:250 diluted in PBS with 0.1% BSA (TPBS also
> works) and original article has 0.05% Tween 20 added to buffer, we
> took it out. Jackson ImmunoResearch was source of latter secondary, and we
> had some of the cleanest staining ever on a rat antimouse inhouse antibody.
>
> The primary is diluted in buffer, but 25ug/ml Rat IgG is added to
> this diluent. The rationale is there will be no cross reaction
> with mouse B cell immunoglobulins, and it works, normal serum blocking
> is not necessary. Dako peroxidase block is recommended by PharminGen,
> avoid methanol. When we use a goat antirat secondary, it is diluted
> in the normal blocking serum (2.5% mouse/5 - 10% goat serum)
>
> For paraffin sections and NO ANTIGEN RETRIEVAL, you can try a
> nonformalin fixative H Nitta et al, Cell Vision, vol 1, no 4, 1997.
> it is zinc chloride, zinc acetate, calcium acetate in TRIS-HCl buffer,
> and gives excellent staining with dilutions comparable to frozen
> section/acetone fixtion. Is a wonderful way of not struggling with
> NBF/fixed mouse tissues. Will be glad to email you the recipe.
>
> Gayle Callis
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 18:12:57 -0600
> From: Carlos Genty <cgenty@bcm.tmc.edu>
> Subject: Beta-Amyloid thanks!
>
> A warm thank you to all who responded to my Beta-amyloid inquiry.
>
> Sincerely,
>
> Carlos Genty
> Baylor College of Medicine
> Dept. of Neurology
> Neuromuscular Pathology Laboratory
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 18:46:19 -0600
> From: "Technical Services" <techserv@dakousa.com>
> Subject: Re: Good waterbath
>
> Jeff,
> I'm not sure what the catalog number is, but the Fisher Isotemp 205 is a
> good
> little waterbath.
>
> Joel Weisenberger
> DAKO Corporation
> Technical Services
> 800-235-5743 x5325
> techserv@dakousa.com
> - -----Original Message-----
> From: Jeff Silverman <peptolab@hamptons.com>
> To: histonet@Pathology.swmed.edu <histonet@Pathology.swmed.edu>
> Date: Tuesday, January 19, 1999 3:28 PM
> Subject: Good waterbath
>
>
> >Netters,
> >Anyone know of a good, inexpensive table top waterbath suitable for heating
> >2 or 3 coplin jars to 95 degrees C for Her2neu antigen retrieval?
> >
> >Jeff Silverman
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 19:15:41 -0600
> From: ALF569@aol.com
> Subject: Re: IHC: ER/PR tissues washing off
>
> Hi Paula-
> We use Poly-l slides and we use Dako target retrieval in a 100 degree
> waterbath for
> 40 min. and we don't have a problem of having the slides wash off.
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 19:25:27 -0600
> From: Cynthia A Delong <DELONG_CYNTHIA_A@LILLY.COM>
> Subject: Isolectin stain
>
> I would like to know if anyone has a protocol for isolectin B4. I have tried
> it several times but it continues to bind to the plaques in mouse brain. Any
> suggestions would be appreciated.
>
> Thank you
> Cindy
> C.DeLong@lilly.com
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 19:35:49 -0600
> From: "tylee" <tylee@itis.com>
> Subject: Re: Antibody Lot Test Question
>
> Histonet,
>
> Customers should be able to assume that vials of antibody that have the
> same lot number will have the same performance upon receipt in the lab. If
> the vendor is reliable, testing of the same lot number of freshly opened
> antibody should not be necessary. Once the vials are opened (thawed,
> aliquots made, or whatever...) it is a different story, so comparison of
> what has been stored in the lab with a fresh vial may give different
> results.
>
> Vendors may have different lot numbers for the same bulk batch of raw
> material. Each dispensed group of tubes (even if they are from the same
> batch) should have a unique traceable lot number.
>
> My 2 cents worth.
>
> Ty Lee
>
> - ----------
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> To: histonet@pathology.swmed.edu <HistoNet@Pathology.swmed.edu>;
> 'sjrugby@juno.com'
> Subject: RE: Antibody Lot Test Question
> Date: Tuesday, January 19, 1999 8:17 AM
>
> Sandosis,
> My understanding of lot numbers is that they are batch numbers.
> Large volume that has been aliquot. Therefore I would not mix different lot
> numbers. I would also not mix the different samples from the same lot. Each
> aliquot has the potential of being subjected to detrimental treatment,
> temperature would be an example. I would check a new aliquot of antibody
> even with the same lot number for the same reason.
> I view lot numbers as a way of checking with the company if I
> happens to have problems at the bench.
>
> Cynthia Favara
> Rocky Mountain Laboratories
> 903 S 4th Street
> Hamilton, MT 59840
> ph: 406-363-9317
> FAX: 406-363-9286
> e-mail: cfavara@nih.gov
>
>
>
> > ----------
> > From: sjrugby@juno.com[SMTP:sjrugby@juno.com]
> > Sent: Friday, January 15, 1999 9:02 PM
> > To: histonet@pathology.swmed.edu
> > Subject: Antibody Lot Test Question
> >
> > Histonetters,
> > A couple of immnu lot testing queries....
> >
> > -How do you feel about mixing primary antibody lots together when there
> > isn't enough of the old lot.
> > Whether you say "Yay" or "Nay", please include your reasoning.
> >
> > -If a new antibody lot arrives in the lab and it has the same lot number
> > as the current tested antibody, is it necessary to test it? Again,
> > please share your reasons for agreeing or disagreeing.
> >
> > -What DO you do with a drunken sailor?
> >
> > Many thanks,
> > Sandosis
> >
> > ___________________________________________________________________
> > You don't need to buy Internet access to use free Internet e-mail.
> > Get completely free e-mail from Juno at http://www.juno.com/getjuno.html
> > or call Juno at (800) 654-JUNO [654-5866]
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 19 Jan 1999 22:30:14 -0600
> From: lastandfirst@webtv.net (Kathleen Hollenbeck)
> Subject: Job Opportunity in Las Vegas
>
>
> We have an opening in our lab for a HT or HTL with at least 5 years of
> experience in a clinical setting. The shift is Wednesday - Sunday 6:30
> - - 3pm. Strong frozen section skills are necessary. Other job duties
> include embedding, cutting and special stains.
>
> Please fax your resume to 702-731-8045 or call Kathleen Hollenbeck,
> Histology Supervisor at 702-731-8050.
>
> Kathleen Hollenbeck
> Histology Supervisor
> Sunrise Hospital
>
>
>
> Here are the messages received yesterday!
>
>
<< Previous Message | Next Message >>