Re: LCM protocols
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| From: | Louise Burrell <lburrell@pathbox.wustl.edu> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Dear Richard---We have an LCM Set up---e-mail me directly and I will fax
you everything I have---The company really should include with your stuff.
Write soon
sincerely
Louise Burrell
Washington U. Sch. of Med. Cancer Center
Tumor Repository Core Laboratory
St. Louis, MO> 63110
On Thu, 21 Jan 1999, HistoNet Server wrote:
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 00:11:01 -0600
> From: Katri Tuomala <katri@istar.ca>
> Subject: Re: Lung Embedding Protocol
>
> sbledsoe wrote:
> >
> > I have been asked to Post the following:
> >
> > Could someone please suggest a good protocol for embedding lung tissue in
> > paraffin.
> >
> > Thanks
> >
> > Sharon B. Bledsoe
> > Indiana University
> > School of Medicine
> > Dept. of Surgery
> > Indianapolis, IN 46202
> > sbledsoe@iupui.ed
> Dear Sharon,
> We don't treat the lung tissue differently from other routine
> processing, when dealing with tumors. If the study however involves
> other lung diseases, we have a protocol to infuse the fresh specimen
> with 10% formalin by a Ghurg(?) method, which I can fax, e-mail, send to
> you, if you are interested. This method distends the lung tissue, so
> that the alveoli can be studied easier. After a proper fixation time,
> the tissue is sectioned and processed normally. Katri.
>
> Katri Tuomala
> Anatomic Pathology
> Sy.Joseph's Hospital
> Hamilton, Ontario, Canada
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 00:24:44 -0600
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Re: silane or plus charge slides
>
> On Tue, 19 Jan 1999, Gayle Callis wrote:
>
> > Interesting comment about deterioration of plus charge slides.
> >
> > I just talked to my Erie Scientific rep, and he said the opposite about
> > silanized slides, that they get better with age, but gave no scientific
> > basis for that statement, any comments? I have always dated
> > poly l lysine slides, since this is a protein, and tried to use them
> > within 6 months.
>
> Both types work by imparting a positive charge to the surface of
> the glass. With silanized slides, amino groups are covalently
> joined to the polymeric SiO2 molecular units of which glass is
> made. Polylysine (there is no reason to use poly-L-lysine;
> poly-DL-lysine is a bit cheaper, though both are expensive) is
> a large molecule bristling with amino groups. Except in
> strongly alkaline conditions, these amino groups are protonated
> (positively charged). This allows them to attract the predominantly
> negative charge of structural proteins of animal tissues. It also
> sticks polylysine to the negatively charged (silicic acid) surface
> of glass.
>
> These charge attractions are easily changed by the
> ambient pH. For silanized slides or polylysine, adhesion to the
> "typical" proteinaceous section will be least effective in an
> alkaline medium. Cartilage is different because its matrix is
> full of sulphate-ester groups, which are strong acids - negative
> at any pH. Cartilage can therefore be expected to adhere more
> securely to a positively charged surface than most other tissues.
>
> Chrome-gelatin is an old-fashioned but excellent adhesive that
> works by forming strong coordinate bonds with oxygen atoms of
> carboxyl groups, which are present in all proteins and in many
> lipids and carbohydrates. It works well in alkaline conditions,
> but fails if strong acids are used - as in some antigen retrieval
> procedures.
>
> > However, the newer lot seems to be the problem. Thanks to the
>manufacturers
> > who put those invaluable lot numbers on products, at least they can see
> > if others are cplaining/having problems.
> > Beginning to think gremlins live in buffers.
>
> It's easy to believe something could go wrong in silanizing a
> huge number of slides. I do my own, by hand, and have had one
> bad batch of 100 (it was the first attempted) in 3 years. They
> were bad only for a pretty severe test that involved heating in
> an alkaline solution. Chrome-gelatin was somewhat better.
>
> Commercial silanized slides seem to be a bit better than those
> we make in the lab, but they are frightfully expensive.
> The commercial silanized slides don't come with a prescription
> for making you own. I suspect that instead of the regular
> published technique they use a reagent that covalently binds
> a quarternary nitrogen (positive charge at any pH) to the glass.
> Our local silicon chemist says this should be better, and has
> advised me about potentially better silanizing compounds.
> Some of these will soon be tested ....
>
> Rather a long comment on a sensible answer to a significant
> question! If you've bothered to read it to the end, then,
> Thanks. Please let me know if you disagree, and why. Email
> has reintroduced the noble tradition of the Letter as a form
> of discourse (our 4 bears would have said "intercourse")
> among scientists. It's unfortunate that the writing and mailing
> of real letters on paper are now too costly for frequent use,
> unless you're sending an advert or a bill.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 00:25:25 -0600
> From: "Mick Rentsch" <ausbio@nex.com.au>
> Subject: Re: Lung Embedding Protocol
>
> If you are only talking about wedge, trucut, or fna biopsies, then your
> routine overnight Paraffin cycle should be quite fine. But if you are doing
> whole lung sections, then I suggest you use a Gelatin technique and/ or
> approach someone from the AFIP in Washington.
> Regards Mike (Downunder)
> - -----Original Message-----
> From: sbledsoe <sbledsoe@iupui.edu>
> To: HistoNet@Pathology.swmed.edu <HistoNet@Pathology.swmed.edu>
> Date: Wednesday, 20 January 1999 2:16
> Subject: Lung Embedding Protocol
>
>
> >I have been asked to Post the following:
> >
> >Could someone please suggest a good protocol for embedding lung tissue in
> >paraffin.
> >
> >
> >
> >Thanks
> >
> >Sharon B. Bledsoe
> >Indiana University
> >School of Medicine
> >Dept. of Surgery
> >Indianapolis, IN 46202
> >sbledsoe@iupui.ed
> >
> >
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 00:49:59 -0600
> From: "Mick Rentsch" <ausbio@nex.com.au>
> Subject: Re: ER/PR tissues washing off
>
> We had the same problem with Poly L Lysine slides (even though kept in
> freezer) we changed over to Silanised slides and have never had a
> recurrence. Please note that we never make up more slides than we can use in
> a month.
> Regards MIke (downunder)
> - -----Original Message-----
> From: Paula Wilder <histo20@hotmail.com>
> To: HistoNet@Pathology.swmed.edu <HistoNet@Pathology.swmed.edu>
> Date: Tuesday, 19 January 1999 1:16
> Subject: IHC: ER/PR tissues washing off
>
>
> >Hello Histonet!
> >
> >Been out of contact with the net for several months, so I hope this
> >query is not a recent duplicate. For some reason, all of a sudden, our
> >ER and PR tissues have been washing off the slide after antigen
> >retrieval using a rice steamer. We use charged slides. We have tried
> >keeping the slides overnight in our incubator oven at 60 degrees. We
> >have thought about incubating the slides in a 75-80 degree oven for a
> >shorter period of time, but have not done so yet.
> >We have also thought about trying poly-L-lysine slides. Do you have any
> >thoughts? Any information will be very greatly appreciated. THANK
> >YOU!!!!
> >
> >
> >Paula Wilder
> >St. Joseph Medical Center
> >Towson, MD
> >
> >______________________________________________________
> >Get Your Private, Free Email at http://www.hotmail.com
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 01:45:59 -0600
> From: Darren Grima <Darren_Grima@clubmac.org.au>
> Subject: Glycerol
>
> I was speaking to a friend of mine, whom works at Liverpool Histo and he told
> me that apparently Glycerol is added to 100% ethanol station or stations in
> the tissue processor. Would anyone know why. I've never heard of this. Cya
>
> Darren
>
> Electron Microscope Unit
> St. George Hospital
> Kogarah NSW Australia
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 03:15:27 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: Good waterbath
>
> >Date: Tue, 19 Jan 1999 17:57:41 -0500
> >From: Jeff Silverman <peptolab@hamptons.com>
> >Subject: Good waterbath
> >To: histonet@pathology.swmed.edu
> >MIME-version: 1.0
> >
> >Netters,
> >Anyone know of a good, inexpensive table top waterbath suitable for heating
> >2 or 3 coplin jars to 95 degrees C for Her2neu antigen retrieval?
> >
> >Jeff Silverman
> >
>
> Jeff,
> I use a spare floating out bath. If you have one, calibrate it, it
> might work.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 03:24:55 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject:
>
> >Date: Tue, 19 Jan 1999 16:00:18 -0700
> >From: Frank Walmsley <frankw@U.Arizona.EDU>
> >To: Histonet@pathology.swmed.edu
> >MIME-version: 1.0
> >
> >Dear histonetters,
> > I will be receiving some mouse skeletal muscle that has been injected
> >with flourescently labelled DNA. It has been frozen in OCT and stored at
> >-80 amd will be frozen sectioned. My question is what would be the best
> >post-treatment of the frozen sections in terms of fixation and
> >coverslipping for either confocal or UV microscopy. Would post fixation in
> >Acetone work or would this affect the flourochrome? Any suggestions would
> >be much appreciated!
> >
> > Frank
>
> Frank,
> I just section, coverslip and ring.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 03:33:56 -0600
> From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
> Subject: Re: Lung Embedding Protocol
>
> >Date: Thu, 21 Jan 1999 00:07:01 -0500
> >From: Katri Tuomala <katri@istar.ca>
> >Subject: Re: Lung Embedding Protocol
> >To: sbledsoe <sbledsoe@iupui.edu>
> >Cc: HistoNet@pathology.swmed.edu
> >MIME-version: 1.0
> >
> >sbledsoe wrote:
> >>
> >> I have been asked to Post the following:
> >>
> >> Could someone please suggest a good protocol for embedding lung tissue in
> >> paraffin.
> >>
> >> Thanks
> >>
> >> Sharon B. Bledsoe
> >> Indiana University
> >> School of Medicine
> >> Dept. of Surgery
> >> Indianapolis, IN 46202
> >> sbledsoe@iupui.ed
> >Dear Sharon,
> >We don't treat the lung tissue differently from other routine
> >processing, when dealing with tumors. If the study however involves
> >other lung diseases, we have a protocol to infuse the fresh specimen
> >with 10% formalin by a Ghurg(?) method, which I can fax, e-mail, send to
> >you, if you are interested. This method distends the lung tissue, so
> >that the alveoli can be studied easier. After a proper fixation time,
> >the tissue is sectioned and processed normally. Katri.
> >
> >Katri Tuomala
> >Anatomic Pathology
> >Sy.Joseph's Hospital
> >Hamilton, Ontario, Canada
> >
>
> Katri,
> If it's no bother, could you put the reply on Histonet. As a
> Histo/Physiologist I would be interested.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 04:00:52 -0600
> From: "Richard Edwards" <REE3@leicester.ac.uk>
> Subject: LASER CAPTURE
>
>
>
> Does anyone have a fixation/processing schedule for preparing tissue
> for
> the laser capture technique, that they woud be able share with me????
>
> Many thanks
>
> Richard
> Edwards......................
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 05:15:58 -0600
> From: Difclt@aol.com
> Subject: Re: HTL examination
>
> I'd like to piggy back under Kimberly's subject and ask if any one knows of a
> computerized study program for HT and HTL? Good luck Kimberly, I too am
> studying right now (for HT).
>
> STEVE
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 05:46:01 -0600
> From: ALLISON@cardiff.ac.uk
> Subject: Steve Slapp
>
> Sorry all.
>
> Steve (Slapp) could you contact me please?
> Russ Allison, Wales
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 06:31:05 -0600
> From: Joanne Schoonmaker <schoonmj@VAX.CS.HSCSYR.EDU>
> Subject: Bone Histomorphometry Courses
>
> Is anyone aware of bone histomorphometry course offerings in 1999 either
> alone or as part of meetings?
>
> Thanks,
> Joanne Schoonmaker
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 06:31:27 -0600
> From: Robert Santoianni <Robert_Santoianni@Emory.Org>
> Subject: silane or plus charge slides -Reply
>
> Our Histo Lab uses "charged" slides for IHC and I use them for frozen
> section immunofluorescence. I can't comment on the longevity of the
> charge because we use them up so fast. But I have had a problem with
> a crosshatch pattern background due to poor QC when they paint the
> label on. We used to use slides from Fisher, but Surgipath gave us a
> better price per case.
> Bob Santoianni
> Emory University Hospital
> Atlanta, Georgia
> robert_santoianni@emory.org
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 07:15:44 -0600
> From: "Barry Rittman" <brittman@mail.db.uth.tmc.edu>
> Subject: Re: Glycerol
>
> Darren,
> I would suspect that the glycerin is there to
> 1. to counteract some of the hardening nd shrinkage that occurs in the
> alcohols and
> 2. as an additive to counteract the evaporation of the alcohols.
> However, glycerol is very expensive and I expect that because of this it is
> rarely used in automatic processors.
> Barry
>
>
> At 10:31 PM 8/27/70 +1100, you wrote:
> >I was speaking to a friend of mine, whom works at Liverpool Histo and he
>told
> >me that apparently Glycerol is added to 100% ethanol station or stations in
> >the tissue processor. Would anyone know why. I've never heard of this. Cya
> >
> >Darren
> >
> >Electron Microscope Unit
> >St. George Hospital
> >Kogarah NSW Australia
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 07:30:42 -0600
> From: "Jim Staruk" <jstaruk@masshistology.com>
> Subject: Re: Good waterbath
>
> We use Teflon-coated skillets (of varying depths), bought at Wall-Mart (or
> any other appliance store). They cost about $30.00 and keep perfectly
> controlled temperatures. We also use these for water baths.
> _________________________
> James E. Staruk, HT(ASCP)
> Mass Histology Service
> http://www.masshistology.com
> - -----Original Message-----
> From: Jeff Silverman <peptolab@hamptons.com>
> To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
> Date: Tuesday, January 19, 1999 6:22 PM
> Subject: Good waterbath
>
>
> >Netters,
> >Anyone know of a good, inexpensive table top waterbath suitable for heating
> >2 or 3 coplin jars to 95 degrees C for Her2neu antigen retrieval?
> >
> >Jeff Silverman
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 08:00:24 -0600
> From: Katie B <bresee98@yahoo.com>
> Subject: Re: Lung Embedding Protocol
>
> - ---sbledsoe <sbledsoe@iupui.edu> wrote:
> >
> > I have been asked to Post the following:
> >
> > Could someone please suggest a good protocol for embedding lung
> tissue in
> > paraffin.
> >
> >
> >
> > Thanks
> >
> > Sharon B. Bledsoe
> > Indiana University
> > School of Medicine
> > Dept. of Surgery
> > Indianapolis, IN 46202
> > sbledsoe@iupui.ed
>
>
> We routinely work with whole lungs from rat and mouse (and sometimes
> the occasional horse lung lobe). The key to getting good sections is
> proper fixation which involves inflating the lung with fixative
> through the trachea so that the avolei can be microscopically
> examined. The proper way to do this is by constant pressure perfusion
> for about an hour for formalin fixation. The most basic version of
> the gizmos we have designed to accomplish this is a seperatory funnel,
> filled with fixative, with tubing attached at the base so that a
> cannulated lung can be attached. The volume in the seperatory funnel
> is kept constant so that the pressure in the lung will be constant.
> Our protocol calls for 20-30 cm of pressure for rat and mouse lungs.
> After fixation we then trim out the desired tissue region then submit
> for routine paraffin embedding.
>
>
>
>
> ==
> Catherine "Katie" Bresee Bennett
> Laboratory for Experimental Pathology
> Department of Veterinary Pathology
> Michigan State University
>
> *new* e-mail: bresee98@yahoo.com
>
> _________________________________________________________
> DO YOU YAHOO!?
> Get your free @yahoo.com address at http://mail.yahoo.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 08:00:48 -0600
> From: Alex Brown <AlexB@nayrshire.scot.nhs.uk>
> Subject: RE: Glycerol
>
> Hi Darren,
> Perhaps it's a similar theory to adding phenol to the alcohol,
> i.e. to prevent the tissue becoming brittle. Haven't heard of using
> glycerol before tho'.
> Alex.
> Kilmarnock, Scotland.
> ----------
> From: Darren Grima
> To: HistoNet Server
> Subject: Glycerol
> Date: 27 August 1956 12:31
>
> I was speaking to a friend of mine, whom works at Liverpool Histo and he
> told
> me that apparently Glycerol is added to 100% ethanol station or stations
> in
> the tissue processor. Would anyone know why. I've never heard of this.
> Cya
>
> Darren
>
> Electron Microscope Unit
> St. George Hospital
> Kogarah NSW Australia
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 08:20:56 -0600
> From: "Ms Louise Taylor" <179LOU@chiron.wits.ac.za>
> Subject: bone
>
> HI guys,
> just a quick question to all you bone buffs out there. I have been
> asked to cut cryostat sections of bone/cartilage interfaces. What is
> the best way to go about this?. I have tried to do this in the dim and
> murky past, and recall that i had trouble with section adherence.
> The cartilage stayed on, but the bone floated free.
> Any suggestions? Also this sound silly, but is it possible to
> decalcify bone without fixation? I want to preserve as natural an
> architecture, but get rid of the minerals?
>
> Thanks in advance
> louise taylor
> SAIMR
> Johannesburg
> SA
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 08:30:12 -0600
> From: Cheryl Crowder <crowder@vt8200.vetmed.lsu.edu>
> Subject: Jan Minshew
>
> Good morning - I'm on jury duty so really I'm not here. But I would like
> the e-mail address and/or phone number for Jan Minshew from Olympus if
> anyone has it. Thank you in advance.
> Cheryl Crowder
> (225) 346-3399
> crowder@vt8200.vetmed.lsu.edu
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 09:01:02 -0600
> From: "Tim Morken" <timcdc@hotmail.com>
> Subject: Re: HTL examination study on computer
>
> The NSH has their study guides on disks. They can be used as a review or
> as a test. It works really well. (http://www.nsh.org/)
> They only work on IBM clones, however.
>
> Tim Morken, B.S., EMT(MSA), HTL(ASCP)
> Infectious Disease Pathology
> Centers for Disease Control
> MS-G32
> 1600 Clifton Rd.
> Atlanta, GA 30333
> USA
>
> email: tim9@cdc.gov
> timcdc@hotmail.com
>
> FAX: (404)639-3043
> - ----Original Message Follows----
> Date: Wed, 20 Jan 1999 05:59:26 -0500 (EST)
> From: Difclt@aol.com
> Subject: Re: HTL examination
> To: brbenson@wyoming.com, histonet@Pathology.swmed.edu
>
> I'd like to piggy back under Kimberly's subject and ask if any one knows
> of a
> computerized study program for HT and HTL? Good luck Kimberly, I too am
> studying right now (for HT).
>
> STEVE
>
>
>
>
>
>
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 09:01:25 -0600
> From: T.Hacker@har.mrc.ac.uk
> Subject: section loss
>
> Just to add to the current slide and lost section debate, I have been
> cutting frozen sections of whole mount ISH stained embryos previously
> fixed and post fixed in paraformaldehyde, infiltrated in glucose and
> embedded/oriented in OCT. When I come to mount them ( aqueous ) any
> attempt to remove the OCT by rinsing in water or PBS results in the
> embryos going for a swim! The only way around this is to mount them
> "dry" resulting in excessive bubble formation.
> I use charged slides, air dry the sections for hours or overnight and
> have even tried "fixing" in formalin vapour to stick the sections to
> the slide but with no success.
> Any ideas?
>
> Terry.
> Terry Hacker,
> Medical Research Council,
> Harwell,
> Oxfordshire,
> U.K.
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 11:04:12 -0600
> From: Cynthia Favara <cfavara@atlas.niaid.nih.gov>
> Subject: RE: LASER CAPTURE
>
> Just curious what is laser capture technique??
> Cynthia Favara
>
> > ----------
> > From: Richard Edwards[SMTP:REE3@leicester.ac.uk]
> > Sent: Wednesday, January 20, 1999 1:51 AM
> > To: HistoNet@Pathology.swmed.edu
> > Subject: LASER CAPTURE
> >
> >
> >
> > Does anyone have a fixation/processing schedule for preparing tissue
> > for
> > the laser capture technique, that they woud be able share with me????
> >
> > Many thanks
> >
> > Richard
> > Edwards......................
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 11:04:49 -0600
> From: "eileen connelly" <leen41@hotmail.com>
> Subject: cpt code for b5
>
> I have checked the CPT 1999 procedure, perhaps I overlooked the code.
> Is there a special fixative CPT code, ie B5, AZF etc,. We can not
> charge patients without this code.
>
> Thanks
> Eileen M. Connelly
> CDH
> Winfield, Ill.
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 11:05:12 -0600
> From: "Mickie L. Johnson" <johnsom@shmc.org>
> Subject: RE: Melan-A antibody from Nova Castra
>
> Hi everyone!
>
> I am interested in your experience with the Melan-A antibody from Nova
> Castra or ? and whether you are using routinely to look at melanomas and
> melanocytes and if any confusing results are seen on non-melanocytic tumors
> and cells. Any info would be appreciated.
> Thanks
>
> Mickie Johnson
> Sacred Heart Medical Center
> Spokane, Washington
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 11:05:34 -0600
> From: "Coskran, Timothy M" <timothy_m_coskran@groton.pfizer.com>
> Subject: EGFR
>
> Hello,
>
> Does anyone have any recommendations for a EGFR clone that works well on
> human and/or mouse tissue?
>
> Thanks in advance
>
> Tim Coskran
> Pfizer
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 11:06:00 -0600
> From: emaher@zeiss.com (Evanne Maher)
> Subject: Re: Microtomes and Carpel Tunnel Syndrome
>
> If you go to the Histotech's Portal (histology.to) under the section
> Peggy's Link to Histology Resources on the WWW one catagory is
> Repetitive Motion Disorder and Latex Allergies. It looks like this
> section is from Leica's web site and it lists articles and ways to
> prevent the problem.
> We sell more of our motorized microtomes for health reasons than for
> cutting of hard tissue. So this is a big problem for employers who
> can't afford to pay when an employee is on disablity.
>
> Evanne Maher, Carl Zeiss Histology Products
>
>
> ______________________________ Reply Separator
> _________________________________
> Subject: Microtomes and Carpel Tunnel Syndrome
> Author: christine lee <C.lee@uq.net.au> at Internet
> Date: 1/16/99 1:10 PM
>
>
> A few years ago I was aware of debate in histology laboratories regarding
> the repeditive stress injuries caused by continued use of microtomes, but
> didn't keep any of the relevant literature. Can anyone refere me to relevant
> literature or websites, or is there anyone who has suffered any repeditive
> strain injuries.
> I would love to hear from you so I can convince our pathologists that it
> does exist. Thanks.
>
> Christine Lee
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 11:06:25 -0600
> From: oshel@terracom.net (Philip Oshel)
> Subject: RE: Glycerol
>
> I think this is the right idea. Glycerol is added to EtOH for preserving
> invertebrates, especially arthropods, just for this reason. It not only
> keeps the specimens from getting brittle, but also prevents them from
> drying out, should the EtOH evaporate. This isn't likely in a histo lab,
> but is almost inevitable in a museum collection.
>
> Phil
>
> >Hi Darren,
> > Perhaps it's a similar theory to adding phenol to the alcohol,
> >i.e. to prevent the tissue becoming brittle. Haven't heard of using
> >glycerol before tho'.
> > Alex.
> > Kilmarnock, Scotland.
>
> >I was speaking to a friend of mine, whom works at Liverpool Histo and he
> >told
> >me that apparently Glycerol is added to 100% ethanol station or stations
> >in
> >the tissue processor. Would anyone know why. I've never heard of this.
> >Cya
> >
> >Darren
> >
> >Electron Microscope Unit
> >St. George Hospital
> >Kogarah NSW Australia
>
> ****be famous! send in a tech tip or question***
> Philip Oshel
> Technical Editor, Microscopy Today
> PO Box 620068
> Middleton, WI 53562
> (608) 833-2885
> oshel@terracom.net
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 13:34:49 -0600
> From: garygill <garygill@dcla.com>
> Subject: Photomicrograph
>
> How does one access the photos? The address given is for a bibliographic
> database. Thanks.
>
> Gary Gill
>
> - -----Original Message-----
> From: Mark & Carrie Byrne [mailto:eire@teleport.com]
> Sent: Tuesday, January 12, 1999 8:25 PM
> To: Luke, Don; histonet
> Subject: RE: PHOTOMICROGRAPH
>
>
> hi don,
> i believe you might be able to find it at IPOX (standford university's IHC
> database). they have an incredible library of photos. here's the address:
> http://ipox.stanford.edu/cgi-bin/ipox
> just click accept and then start and it puts you at the login
> screen.....username and password are both "guest".
> hope you find what you need...
> carrie kyle-byrne
> eire@teleport.com
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 13:35:14 -0600
> From: garygill <garygill@dcla.com>
> Subject: Lost sections ER/PR
>
> The persistence of a problem despite many "solutions" is often an indication
> that the underlying fundamental cause has not been identified. In the case
> of tissue sections adhesion is promoted by mutually attractive surfaces
> (i.e., opposite electrical charges) in contact with one another. Tissue and
> glass are naturally attractive under the right conditions. Specifically,
> the glass must be squeaky clean and the tissue must lie flat against the
> glass. Folds can contract during fixation and help separate the sections.
>
> To make glass wettable, simply immerse slides in alcohol and wipe dry with
> clean cheesecloth immediately before use. Precleaned and/or coated glass
> will naturally adsorb electrical atmospheric/particulate charges over time
> and become less wettable (hence Jeff's remark that "Positive-charged slides
> can deteriorate with age"). Using this method in cytology results in good
> cell recovery and flattening. This method is better than frosted slides,
> albuminized slides, albuminized cell buttons, poly-L-lysine coated slides,
> etc. BTW, moisture that naturally condenses between packed slides over time
> interacts with the silica and forms HCl, which etches the glass and leaves
> the whitish deposit we see, furthering reducing attractive forces. Thus,
> Clay Adams began packaging its slides in hermetically sealed wrap with
> silica gel packed inside many years ago. Cover glass manufacturers followed
> suit and include small packets in the 1-oz plastic containers.
>
> Gary Gill
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 13:35:38 -0600
> From: garygill <garygill@dcla.com>
> Subject: FW: PHOTOMICROGRAPH
>
>
> How does one access the photos? The address given is for a bibliographic
> database. Thanks.
>
> Gary Gill
>
> - -----Original Message-----
> From: Mark & Carrie Byrne [mailto:eire@teleport.com]
> Sent: Tuesday, January 12, 1999 8:25 PM
> To: Luke, Don; histonet
> Subject: RE: PHOTOMICROGRAPH
>
>
> hi don,
> i believe you might be able to find it at IPOX (standford university's IHC
> database). they have an incredible library of photos. here's the address:
> http://ipox.stanford.edu/cgi-bin/ipox
> just click accept and then start and it puts you at the login
> screen.....username and password are both "guest".
> hope you find what you need...
> carrie kyle-byrne
> eire@teleport.com
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 14:19:52 -0600
> From: "Norton, Sally" <snort1@chmc.org>
> Subject: RE: subscribe
>
>
>
> Please unsubscribe me. thank you
>
>
> > -----Original Message-----
> > From: HistoNet Server [SMTP:HistoNet@Pathology.swmed.edu]
> > Sent: Friday, January 08, 1999 09:56
> > To: Norton, Sally
> > Subject: re: subscribe
> >
> > Your address has been added to the addresses that comprise this Listserv
> > List.
> > Welcome to HISTONET. This is an electronic mailing list for the exchange
> > of
> > information pertaining to histotechnology and related fields.
> >
> > PLEASE SAVE THIS MESSAGE.
> > It contains useful information about how to use the list and what to do if
> > you
> > experience problems. It also includes some basic rules for email etiquette
> > (Netiquette) which will be helpful to those who are new to this form of
> > communication.
> >
> > WHAT IS A LISTSERVER?
> > A list server is a computer that runs software which will receive incoming
> > electronic mail (email) messages and reroute them automatically to
> > everyone on
> > the subscriber list. Email uses the vast expanse of the Internet to allow
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> > (California) and can currently send about 30 messages a minute. With the
> > present number of subscribers, we are processing about 10,000 outbound
> > messages a day.
> >
> > WHO SHOULD SUBSCRIBE?
> > Anyone interested in research or clinical applications of histology,
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> > microscopy
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> > WHO RUNS HISTONET?
> > The list is run by Linda R. Margraf, M.D. and Herb K. Hagler, Ph.D. using
> > hardware and software owned by the University of Texas Southwestern
> > Medical
> > School, Department of Pathology in Dallas, Texas. If you have any
> > questions or
> > problems with Histonet please contact Linda Margraf at
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> >
> > HOW DOES THE LIST WORK?
> > This server, unlike many systems, uses ONLY ONE ADDRESS to send commands
> > to
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> > listed below. Anything not identified as a command will be circulated to
> > EVERYONE on the list.
> >
> > The following is a list of commands the server recognizes:
> >
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> > Your address will be added to the list of subscribers. You will then be
> > able
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> > subscribers.
> >
> > subscribe digest
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> > digest
> > instead of each forwarded message. A digest is a compilation of all the
> > messages received in a 24 hour period. It is sent to the digest
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> > every night after midnight. Digest subscribers can post and respond to
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> >
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> > A list of the commands recognized by the server will be returned to
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> >
> > WHAT ARE THE RULES?
> > You may post any questions you wish pertaining to histology, pathology,
> > in-situ hybridization, immunohistochemistry etc. Equipment and reagent
> > evaluations, laboratory management issues, government regulations, and job
> > opportunities are all appropriate topics. The University asks that we
> > restrict
> > the use of its hardware and software to business purposes only (occasional
> > jokes do slip through but PLEASE use restraint). Vendors and those with
> > commercial interests in histology products are welcome contributors
> > however,
> > we ask that blatant advertisements be avoided at all times. It is fine to
> > refer to product that your company produces if it is pertinent to a topic
> > being discussed on the list. Unsolicited advertisements are poorly
> > tolerated
> > by the members and you will likely receive a number of negative comments
> > if
> > you overstep the boundaries. Please contact Linda Margraf at
> > LMargraf@childmed.dallas.tx.us if you are not sure about the
> > appropriateness
> > if a message you wish to post.
> >
> >
> > BASIC HISTONET "NETIQUETTE"
> > It is most helpful to the list members if you post your responses to
> > queries
> > to everyone on the list and not just as a personal reply to the person
> > asking
> > the question. That way duplicate messages are minimized and we all learn
> > from
> > each other's comments.
> >
> > Likewise, if you post a question and get a number of responses back
> > directly
> > to you, it is helpful to everyone if you could send out a summary of the
> > replies you got to Histonet.
> >
> > Please avoid abbreviations unless they are explained in your message. For
> > example: immunohistochemistry (IHC). This list circulates to a wide
> > variety of
> > individuals and what seems obvious to you may have no meaning on the other
> > side of the world.
> >
> > Please sign your letter and include your institution or affiliation and
> > location. Not all email systems have headers which identify the sender.
> >
> > Do use the subject line to indicate the topic of your message.
> >
> > DON'T USE ONLY CAPITAL LETTERS -it is considered shouting.
> >
> > Please send questions and problems about the list directly to Linda
> > Margraf at
> > LMargraf@childmed.dallas.tx.us and don't circulate them to the >850
> > subscribers on the list. Be careful when sending commands to the server to
> > put
> > the command in the SUBJECT LINE and spell it correctly.
> >
> > Please do not send images as attachments with your message. We can now
> > post
> > images at our web site (http://pathcuri1.swmed.edu). To have an image
> > posted
> > send it to Herb Hagler at herb.hagler@email.swmed.edu.
> >
> >
> >
> >
> > es
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 14:21:27 -0600
> From: rkline@emindustries.com
> Subject: Re: Glycerol
>
> Darren,
>
> I would have to agree with other reponses. Glycerol would act as a
> moisturized to keep the tissues from drying out.
>
> Rande Kline HT (ASCP)
> Technical Services
> EM Science
>
>
>
>
> Darren Grima <Darren_Grima@clubmac.org.au> on 08/27/56 07:31:36 AM
>
> To: HistoNet Server <HistoNet@Pathology.swmed.edu>
> cc:
> Subject: Glycerol
>
>
>
>
> I was speaking to a friend of mine, whom works at Liverpool Histo and he
> told
> me that apparently Glycerol is added to 100% ethanol station or stations in
> the tissue processor. Would anyone know why. I've never heard of this. Cya
>
> Darren
>
> Electron Microscope Unit
> St. George Hospital
> Kogarah NSW Australia
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 14:22:55 -0600
> From: "Susan Meloan" <SMELOAN@mail.mcg.edu>
> Subject: Re: HTL examination -Reply
>
> BOR has a study guide which includes sample tests on computer disk. NSH has
> computer disks to accompanied some of the self assessment books.
>
> Susan
>
> >>> <Difclt@aol.com> 01/20/99 05:59am >>>
> I'd like to piggy back under Kimberly's subject and ask if any one knows of a
> computerized study program for HT and HTL? Good luck Kimberly, I too am
> studying right now (for HT).
>
> STEVE
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 15:07:02 -0600
> From: vdella@path.som.sunysb.edu
> Subject: Michael Frederickson/Cliff Chapman
>
> Does anyone have email addresses for Michael Frederickson at Mass.
> General and Cliff Chapman?
>
> please respond off list to avoid inconvenience to others.
> thanks
> ******************************************************************
> Vinnie Della Speranza
> Technical Director
> Anatomic Pathology Laboratories
> University Hospital & Medical Center
> State University of New York at Stony Brook 11794-7025
> (516) 444-8249
> fax: (516) 444-3419
> vdella@path.som.sunysb.edu
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 15:07:54 -0600
> From: garygill <garygill@dcla.com>
> Subject: RE: Glycerol
>
> Glycerol is a trihydric alcohol (i.e., has three hydroxyl groups), and among
> other things, is used as a humectant in the manufacture of chocolate candies
> to keep their surface appearance looking yummy. It has also been used in
> cytology at a 50% concentration to rehydrate air-dried cells so they regain
> their dye penetrability and appear normochratic in the Pap stain. However,
> it can not reverse the changes in cell size and chromatin appearance that
> accompany air-drying.
>
> Gary Gill
>
> - -----Original Message-----
> From: rkline@emindustries.com [mailto:rkline@emindustries.com]
> Sent: Wednesday, January 20, 1999 1:51 PM
> To: Darren Grima
> Cc: HistoNet Server
> Subject: Re: Glycerol
>
>
> Darren,
>
> I would have to agree with other reponses. Glycerol would act as a
> moisturized to keep the tissues from drying out.
>
> Rande Kline HT (ASCP)
> Technical Services
> EM Science
>
>
>
>
> Darren Grima <Darren_Grima@clubmac.org.au> on 08/27/56 07:31:36 AM
>
> To: HistoNet Server <HistoNet@Pathology.swmed.edu>
> cc:
> Subject: Glycerol
>
>
>
>
> I was speaking to a friend of mine, whom works at Liverpool Histo and he
> told
> me that apparently Glycerol is added to 100% ethanol station or stations in
> the tissue processor. Would anyone know why. I've never heard of this. Cya
>
> Darren
>
> Electron Microscope Unit
> St. George Hospital
> Kogarah NSW Australia
>
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 15:36:27 -0600
> From: Joanne Schoonmaker <schoonmj@VAX.CS.HSCSYR.EDU>
> Subject: Diane Sterchi's address
>
> Doe's anyone have Diane Sterchi's email address or telephone number please.
>
> TIA,
> Joanne Schoonmaker
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 15:36:56 -0600
> From: "John C. Dennis" <dennijc@vetmed.auburn.edu>
> Subject: Re: EGFR
>
> Tim
>
> Not precisely what you're wanting BUT I've been using Sigma's anti-human
> EGF-R on paraffin-embedded paraformaldehyde-fixed rat tissue. It has been
> working really well or at least until last weekend. Sigma lists several
> and the one I've used that works for IHC is cat#: E-3138.
>
> John Carroll Dennis
> Anatomy, Physiology, and Pharmacology
> 109 Greene Hall
> Auburn University, AL 36849
>
>
> On Wed, 20 Jan 1999, Coskran, Timothy M wrote:
>
> > Hello,
> >
> > Does anyone have any recommendations for a EGFR clone that works well on
> > human and/or mouse tissue?
> >
> > Thanks in advance
> >
> > Tim Coskran
> > Pfizer
> >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 16:04:17 -0600
> From: rkline@emindustries.com
> Subject: Re: silane or plus charge slides -Reply
>
> Robert,
>
> The problems I am hearing from all the correspondense could possibly be a
> manufacturing problem with the coated slides. One way to find the cause
> of the problem would be to report the problem to the distributor or
> manufacturer and request a new lot of slides as a replacement. If you are
> still having problems with the new lot, most likely it's application. If
> your not, it could possibly be the lot.
>
> The QC end would be making sure the slides meet certain specifications.
> It would be the manufacturing end that paint the label on. Stuff can happen
> during manufacturing and the manufacturer needs the heads-up. Switching
> vendors is not always the solution. Somewhere up the road the problem
> will most likely repeat itself.
>
> Hope this information helps.
>
> Regards,
> Rande Kline HT (ASCP)
> Technical Services
> EM Science
>
>
>
>
> Robert Santoianni <Robert_Santoianni@Emory.Org> on 01/20/99 06:25:14 AM
>
> To: Histonet@pathology.swmed.edu
> cc:
> Subject: silane or plus charge slides -Reply
>
>
>
>
> Our Histo Lab uses "charged" slides for IHC and I use them for frozen
> section immunofluorescence. I can't comment on the longevity of the
> charge because we use them up so fast. But I have had a problem with
> a crosshatch pattern background due to poor QC when they paint the
> label on. We used to use slides from Fisher, but Surgipath gave us a
> better price per case.
> Bob Santoianni
> Emory University Hospital
> Atlanta, Georgia
> robert_santoianni@emory.org
>
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 16:04:52 -0600
> From: "Jennings-Siena, Debbie" <ds.jennings-siena@baylordallas.edu>
> Subject: Need used equipment
>
> Hi,
> An acquaintance asked me to post this on the net, they are in need of some
> used equipment, an embedding center, microscope, processor and microtome.
> If anyone can help with this request please contact me off the air. Thanks
> Debbie J. Siena, HT(ASCP)QIHC
> Baylor University Medical Center
> 3500 Gaston Ave.
> Dallas, Texas
> 214/820-2465 VM
> 214/820-4110 Fax
> ds.jennings-siena@baylordallas.edu
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 16:05:36 -0600
> From: "Barry Rittman" <brittman@mail.db.uth.tmc.edu>
> Subject: Re: Lung Embedding Protocol
>
> Katie,
> there have been several approaches to processing lung and
> ensuring that the reagents are not excluded by the air in the bronchial
> tree.
> One approach was to use fixatives with low surface tension such as alcohol
> containing fixatives or to add a small amount of surfactant such as
> triton X 100 to the fixative.
> I would agree with Sharon that a controlled positive pressure helps in the
> process but would recommend that you also include 0.01% -0.1% surfactant
> in the fixative. This has been shown to decrease surface tension without
> compromising routine histology. I am not sure if this has been used with
> IHC but would be interested to hear some comments about this.
> Another approach is to gently vacuum the fixative before use and use
> gentle vacuum to remove the air, once in the fixative.
> I think that it is also advisable to vacuum infiltrate during paraffin
> processing.
> Barry
>
> Katie B wrote:
>
> > ---sbledsoe <sbledsoe@iupui.edu> wrote:
> > >
> > > I have been asked to Post the following:
> > >
> > > Could someone please suggest a good protocol for embedding lung
> > tissue in
> > > paraffin.
> > >
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 16:06:00 -0600
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> Subject: frozen section loss/slide evaluation
>
> I ran two lots of plus charge slides, side by side, with frozen
> sections, same results with both lots. However, sections were air
> dried longer, over silica gel, fixed in fresh acetone, air dried and
> stored in -80C freezer overnight in sealed box, with bags of silica gel,
> in a ziplock baggie containing a large bag of silica gel (beginning
> to dislike that blue stuff, somewhat gel'd out!)
>
> Sections were greatly improved, did not have the bubble effect artifact,
> but some sections looked strung out, didn't make much difference which
> slide lot.
> This leads me to some conclusion that our humidity (much higher this
> year, due to warmer weather/moist storms) is affecting the sections,
> culprit is moisture! Montana is normally extremely dry in the winter,
> this year is an exception, with humidity in the 30's, and I have a cooler
> lab since they readjusted the theromstat. Back to a hot room! and I may
> have to store my acetone over anhydrous calcium chloride, decant and use.
> Has anyone done this?
>
> I vote for moisture in my case and cannot blame my beloved plus charge
> slides.
>
> Gayle Callis
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 17:00:22 -0600
> From: Carla_Aiwohi@usgs.gov (Carla Aiwohi)
> Subject:
>
> I have a protocol which calls for 1% tartrazine in cellusolve. Can anyone
> tell
> me what cellusolve is? Is there anything I can use instead of cellusolove.
> Thanks in advance,
> Carla Aiwohi
> Western Fisheries Research Center
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 17:00:44 -0600
> From: Carla_Aiwohi@usgs.gov (Carla Aiwohi)
> Subject: cellusolve
>
> I have a protocol which calls for 1% tartrazine in cellusolve. Can anyone
> tell
> me what cellusolve is? Is there anything I can use instead of cellusolove.
> Thanks in advance,
> Carla Aiwohi
> Western Fisheries Research Center
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 17:01:46 -0600
> From: larisonk@UONEURO.uoregon.edu
> Subject: T3/T4
>
> Histonetters,
>
> A graduate student is interested in determining if there is thyroxine in her
> embryonic fish. She has purchased some anti-T3 and -T4 antibodies and is
> wondering if these can be used to detect the thyroxine hormone in sections.
> If
> so, what fixation do people use? I assume the amine-containing
>thyroxine can
>
> be fixed with an aldehyde, but are some aldehyde-containing fixatives better
> than others since this is such a small molecule? And is antigen retrieval
> required?
>
> Thanks so much. Love the net!
>
> Karen Larison - University of Oregon
>
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 17:02:17 -0600
> From: N Kenneison <nakenneison.demon.co.uk@nakenneison.demon.co.uk>
> Subject: Dublin Device
>
> Hi
> Do any of our colleages in Oz know of the Dublin Device for Breast
> Biopsy work and who supplies them.
> Also any comments from users as to their advantages / disadvantages.
> Thanks very much.
> - --
> N Kenneison
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 17:55:05 -0600
> From: "Tim Morken" <timcdc@hotmail.com>
> Subject: Thioflavin S references needed
>
>
> Does anyone have references for the original article(s) concerning
> staining amyloid with "Thioflavin S"?
>
>
> Tim Morken, B.S., EMT(MSA), HTL(ASCP)
>
> Infectious Disease Pathology
>
> Centers for Disease Control
>
> MS-G32
>
> 1600 Clifton Rd.
>
> Atlanta, GA 30333
>
> USA
>
>
>
> email: tim9@cdc.gov
>
> timcdc@hotmail.com
>
>
>
> FAX: (404)639-3043
>
>
>
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 17:56:03 -0600
> From: Philip Seifert <paseifer@MIT.EDU>
> Subject: Re:
>
> Carla,
>
> Cellosolve is ethylene glycol monoethyl ether, its available through Sigma
> Chem. Co.. We used to use it deplasticize methyl methacrylate resin
> sections.
>
> Philip Seifert
> MIT-Biomedical Engineering Center
>
>
>
> At 02:16 PM 1/20/99 -0700, Carla Aiwohi wrote:
> >I have a protocol which calls for 1% tartrazine in cellusolve. Can anyone
> tell
> >me what cellusolve is? Is there anything I can use instead of cellusolove.
> >Thanks in advance,
> >Carla Aiwohi
> >Western Fisheries Research Center
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 20:00:43 -0600
> From: "Hagerty, Marjorie A." <mhagerty@emc.org>
> Subject: Cytochemical charges
>
> Hi Everyone,
>
> Would anyone be willing to share with me what they charge for the
> technical component on cytochemical stains on bone marrow such as
> specific and non-specific esterases, etc.(CPT Code:88319)?
>
> Thanks!
>
> Marg
> EMC, Rancho Mirage, CA, USA
>
>
> ----------------------------------------------------------------------
>
> Date: 20 Jan 1999 21:15:46 -0600
> From: RICHELLE F LONGO <rlongo1@juno.com>
> Subject: Un-subscribe
>
> Please unsubscibe me at this time.
> Thank You
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> Date: 20 Jan 1999 22:31:00 -0600
> From: Katri Tuomala <katri@istar.ca>
> Subject: Lung embedding protocol
>
> Hi all!
> We also have a gizmo set up for perfusing whole human lungs, similar to
> Katie's in Michigan. Following protocol is ment to deal with open lung
> biopsies to diagnose lung diseases:
>
> Reference: Churg A: An inflation procedure for open lung biopsies.
> American Journal of Surgical Pathology 7:69-71, 1983.
>
> The portion of the lung biopsy to be processed to paraffin is distended
> with formalin using 25 gauge butterfly needle connected to a 5 ml
> syringe filled with formalin. After distension the specimen is immersed
> in formalin and allowed to fix at least 30 minutes. All the tissue is
> then cut into blocks and submitted for histology paraffin processing.
>
> I hope this is helpful for all interested. Katri.
>
> Katri Tuomala
> Anatomic Pathology
> St.Joseph's Hospital
> Hamilton, Ontario, Canada
>
>
> Here are the messages received yesterday!
>
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