Re: CD4 and CD8 IHC

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From:Jamie Erickson <> (by way of histonet)
To:histonet <>
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               I stain for CD4; CD8 routinely in mouse spleen a normal
mouse spleen is loaded w/ both. So far only frozens work. Cryosection then
acetone fixed for 1 minute then stored at -20 til stained. I use a techmate
autostainer from ventanna. The antibodies are from Pharmingen CD4 cat
#01071D, 1ug/ml--working Conc, CD8a 01041D, 5ug/ml--working Con. Very easy
very nice staining.

Jamie Erickson
Genetics Institute

>>> "Bourassa, Patricia" <> 01/19 3:26
>>>PM >>>
Hi, all!

Has anyone stained for CD4 and CD8 in mouse tissue?  I was wondering what
conditions people have found to be effective.  How are the levels in spleen?




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