Patricia,
               I stain for CD4; CD8 routinely in mouse spleen a normal
mouse spleen is loaded w/ both. So far only frozens work. Cryosection then
acetone fixed for 1 minute then stored at -20 til stained. I use a techmate
autostainer from ventanna. The antibodies are from Pharmingen CD4 cat
#01071D, 1ug/ml--working Conc, CD8a 01041D, 5ug/ml--working Con. Very easy
very nice staining.

Jamie Erickson
Genetics Institute


>>> "Bourassa, Patricia" <patricia_bourassa@groton.pfizer.com> 01/19 3:26
>>>PM >>>
Hi, all!

Has anyone stained for CD4 and CD8 in mouse tissue?  I was wondering what
conditions people have found to be effective.  How are the levels in spleen?

Thanks.

-Patti


!