Re: Mounting media

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From:"Karen D. Larison" <LARISONK@UONEURO.uoregon.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Cindi,

You might try Mowiol from Calbiochem.  You have to make it up yourself from a
polyvinyl alcohol solid.  They give you the protocol.  It's supposed to
prevent
photobleaching, although I've found that this depends alot on the fluorophore
used.  It takes about a day to dry.

Karen Larison - University of Oregon


Date:          Mon, 18 Jan 1999 11:25:46 -0500 (EST)
From:          Cindipqr@aol.com
Subject:       Mounting media
To:            histonet@Pathology.swmed.edu

Hello Everyone,
	I need some recommendations for a mounting media.  It needs to be water
soluble for 100 um. sections of immunofluorescence.  The media I'm using now
doesn't always dry so the coverslips are moving.  This is quite a problem for
confocal microscopy.
Thanks in advance,
Cindi




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