Re: Mounting media
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From: | "Karen D. Larison" <LARISONK@UONEURO.uoregon.edu> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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Content-Type: | text/plain; charset="us-ascii" |
Cindi,
You might try Mowiol from Calbiochem. You have to make it up yourself from a
polyvinyl alcohol solid. They give you the protocol. It's supposed to
prevent
photobleaching, although I've found that this depends alot on the fluorophore
used. It takes about a day to dry.
Karen Larison - University of Oregon
Date: Mon, 18 Jan 1999 11:25:46 -0500 (EST)
From: Cindipqr@aol.com
Subject: Mounting media
To: histonet@Pathology.swmed.edu
Hello Everyone,
I need some recommendations for a mounting media. It needs to be water
soluble for 100 um. sections of immunofluorescence. The media I'm using now
doesn't always dry so the coverslips are moving. This is quite a problem for
confocal microscopy.
Thanks in advance,
Cindi
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