>Date: Tue, 19 Jan 1999 16:00:18 -0700
>From: Frank Walmsley <frankw@U.Arizona.EDU>
>To: Histonet@pathology.swmed.edu
>MIME-version: 1.0
>
>Dear histonetters,
>     I will be receiving some mouse skeletal muscle that has been injected
>with flourescently labelled DNA.  It has been frozen in OCT and stored at
>-80 amd will be frozen sectioned.  My question is what would be the best
>post-treatment of the frozen sections in terms of fixation and
>coverslipping for either confocal or UV microscopy. Would post fixation in
>Acetone work or would this affect the flourochrome? Any suggestions would
>be much appreciated!
>
>						Frank

Frank,
	I just section, coverslip and ring.
Ian.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Glasgow,
G12 8QQ,
Scotland.
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk