Re: Lung Embedding Protocol

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From:Ian Montgomery <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

>Date: Thu, 21 Jan 1999 00:07:01 -0500
>From: Katri Tuomala <>
>Subject: Re: Lung Embedding Protocol
>To: sbledsoe <>
>MIME-version: 1.0
>sbledsoe wrote:
>> I have been asked to Post the following:
>> Could someone please suggest a good protocol for embedding lung tissue in
>> paraffin.
>> Thanks
>> Sharon B. Bledsoe
>> Indiana University
>> School of Medicine
>> Dept. of Surgery
>> Indianapolis, IN 46202
>> sbledsoe@iupui.ed
>Dear Sharon,
>We don't treat the lung tissue differently from other routine
>processing, when dealing with tumors. If the study however involves
>other lung diseases, we have a protocol to infuse the fresh specimen
>with 10% formalin by a Ghurg(?) method, which I can fax, e-mail, send to
>you, if you are interested. This method distends the lung tissue, so
>that the alveoli can be studied easier. After a proper fixation time,
>the tissue is sectioned and processed normally. Katri.
>Katri Tuomala
>Anatomic Pathology
>Sy.Joseph's Hospital
>Hamilton, Ontario, Canada

	If it's no bother, could you put the reply on Histonet. As a
Histo/Physiologist I would be interested.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
G12 8QQ,
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.

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