-----Original Message-----
From: Alonso Martinez-Canabal [mailto:alonso.martinezcanabal@utoronto.ca] 
Sent: January-07-08 1:27 PM
To: 'histonet-bounces@lists.utsouthwestern.edu'
Subject: MEthanol-free floating



   Hi, 
       I normally work with free floating IHC In brain tissue. Normally I
quench peroxidase activity with H2O2 0.3% in PBS during 30min. Allegledy,
even 5min in the normal 5% concentration would cause damage because free
floating sections are a little bit brittle. But what about the use with
methanol to protect the tissue? I heard that methanol is used to prevent
tissue damage can it be used with free floating sections?
 Thank you
 Alonso 


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