Re: [Histonet] One more thing regarding Luxol Fast Blue differentiator

From:Gayle Callis

Does anyone have a reference for using this differentiator?  I have always 
used the lithium differentiator step with great success and also batch 

At 07:25 AM 1/17/2007, you wrote:
>Hi Everyone:
>In regards to the Luxol Fast Blue differentiator that I use (1% 
>hydroquinone, 5% sodium sulfite aqueous) I forgot to mention that you can 
>simply batch the slides through this solution (in fact through the entire 
>protocol). (I use 60 slide racks). You do not have to differentiate them 
>one at a time.
>Tim Wheelock
>Harvard Brain Tissue Resource Center

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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