This is my big beef with CAP. When I was at the AFIP from 1986-1991, there 
were frozen antibodies that were aliquoted in 1982 at -70 C. When I left in 
1991, we were still using those antibodies with excellent results.
    Then the question came up about revalidating the reagents. When I went 
through a CLIA inspection, the inspector cited me. I told her that every 
time I run a patient, there is always a known positive control run with the 
patient. If it didn't work, then I would repeat it.
    It was tough explaining  that to her because she was long time Chemistry 
tech, which is understandable, but give me a break.
    Is it Friday yet?

Joe
----- Original Message ----- 
From: "Patricia Karlisch" 
To: "Hazel V Horn" ; "Eric Breisch" 
; 
Sent: Wednesday, January 10, 2007 8:28 AM
Subject: RE: [Histonet] CAP regulations regarding storage of antibodiesin 
frost free refrigerator freezers


>A long time ago I was told that you can NOT use a freezer to store
> antibodies or tissue unless it is NON- frost free.  CAP actually cited
> those with frost free due to the temperature declining enough to melt
> ice and thus affect the storage of such samples.  Pat
>
> Pat Karlisch
> Supervisor, Histology, Pathology and Laboratory Medicine
> Penn State Milton S. Hershey Medical Center
> Mail Code H179
> Hershey, PA 17033
> Phone (717) 531-6072
> Fax: (717) 531- 7741
> email: pkarlisch@psu.edu
>
> *****E-Mail Confidentiality Notice*****
> This message (including any attachments) contains information intended
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>
>
>>>> "Horn, Hazel V"  1/10/2007 9:10 AM >>>
>
> I'm don't know of a CAP regulation but it isn't a good idea to store
> antibodies or tissue for that matter in a frost free freezer.   It can
> dry out the antibodies or tissue if left for long periods of time.
>
> Hazel Horn
> Hazel Horn, HT/HTL (ASCP)
> Supervisor of Histology
> Arkansas Children's Hospital
> 800 Marshall    Slot 820
> Little Rock, AR   72202
>
> phone   501.364.4240
> fax        501.364.3912
>
> visit us on the web at:    www.archildrens.org
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> Breisch,
> Eric
> Sent: Tuesday, January 09, 2007 6:21 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] CAP regulations regarding storage of antibodies in
> frost free refrigerator freezers
>
> Hi Histonet-
>
>
>
>            Is anyone aware of new or existing CAP regulations
> regarding
> the use of frost free refrigerator/freezers for the storage of
> antibodies ie. IFA antibodies or IHC antibodies? Please advise if
> anyone
> has information pertaining to Histology and the above mentioned use of
> a
> refrigerator/freezer.
>
>
>
> Thank you,
>
>
>
>
>
> Eric A. Breisch, Ph.D.
>
> Clinical Anatomist
>
> Rady Children's Hospital and Health Center
>
> Associate Clinical Professor of Anatomy
>
> UCSD SOM
>
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