RE: [Histonet] CAP regulations regarding storage of antibodiesin frost free refrigerator freezers

From:"Patricia Karlisch"

A long time ago I was told that you can NOT use a freezer to store
antibodies or tissue unless it is NON- frost free.  CAP actually cited
those with frost free due to the temperature declining enough to melt
ice and thus affect the storage of such samples.  Pat
Pat Karlisch
Supervisor, Histology, Pathology and Laboratory Medicine
Penn State Milton S. Hershey Medical Center
Mail Code H179
Hershey, PA 17033
Phone (717) 531-6072
Fax: (717) 531- 7741
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>>> "Horn, Hazel V"  1/10/2007 9:10 AM >>>

I'm don't know of a CAP regulation but it isn't a good idea to store
antibodies or tissue for that matter in a frost free freezer.   It can
dry out the antibodies or tissue if left for long periods of time.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:

-----Original Message-----
[] On Behalf Of
Sent: Tuesday, January 09, 2007 6:21 PM
Subject: [Histonet] CAP regulations regarding storage of antibodies in
frost free refrigerator freezers

Hi Histonet-

            Is anyone aware of new or existing CAP regulations
the use of frost free refrigerator/freezers for the storage of
antibodies ie. IFA antibodies or IHC antibodies? Please advise if
has information pertaining to Histology and the above mentioned use of

Thank you,

Eric A. Breisch, Ph.D.

Clinical Anatomist

Rady Children's Hospital and Health Center

Associate Clinical Professor of Anatomy


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