Re: [Histonet] optimal thickness for cutting of IHC sections
Sandy:
A 5 µm thichness is the general standard for most laboratories and we used it, BUT
(there is always a "but" isn't it) if we were targeting a nuclear epitone we tried a
little thicker (6-7 µm) and for cytoplasmic ones we tried somewhat thinner (ca. 4 µm).
This approach was also used for histochemistry procedures: at least 6-7 µm for
reticulum staining in bone marrows.
I hope this will help you!
René J.
"Harrison, Sandra C." wrote:
Hi Histonetters,
Is 5 microns the norm for IHC sections? What is considered optimal?
Thanks,
Sandy
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