Re: [Histonet] optimal thickness for cutting of IHC sections

From:Rene J Buesa

Sandy:
  A 5 Ám thichness is the general standard for most laboratories and we used it, BUT
  (there is always a "but" isn't it) if we were targeting a nuclear epitone we  tried a 
  little thicker (6-7 Ám) and for cytoplasmic ones we tried somewhat thinner (ca. 4 Ám).
  This approach was also used for histochemistry procedures: at least 6-7 Ám for
  reticulum staining in bone marrows.
  I hope this will help you!
  RenÚ J.

"Harrison, Sandra C."  wrote:
  Hi Histonetters,

Is 5 microns the norm for IHC sections? What is considered optimal?

Thanks,

Sandy

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