I recently used the kit from Chemicon cat# S7101 ( Looks just like the old
Intergen one to me) and ran caspase 3 along side with near perfect
correlation.I was thrilled because I recalled Tunnel as a troublesome
assay...I bet its been 5 years since I ran it.I just followed the insert and
had no problems.

I'd wish you good luck , but you won't need it!

Sue Wells

Hi Patsy -

I use chemicon [a division of serologicals] #S7101.

It's a little pricey, but works well. I am willing to share my optimized
protocol with you if you choose this one. I do it several times a month for
various investigators. Most have no trouble distinguishing necrosis from
apoptosis... They look for nuclear "blebbing" and fragmented nuclei, etc.
Most requests come from people who have been doing this a long time. [I'm
glad I don't have to do this part] Please let me know if you have any
questions.

Best Regards,

Mary Vaughan HT (ASCP)

 
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg@ihctech.net
web site www.ihctech.net  
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.

 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet