[Histonet] Tunnel responses from IHCRG

From:"Patsy Ruegg"

I recently used the kit from Chemicon cat# S7101 ( Looks just like the old
Intergen one to me) and ran caspase 3 along side with near perfect
correlation.I was thrilled because I recalled Tunnel as a troublesome
assay...I bet its been 5 years since I ran it.I just followed the insert and
had no problems.

I'd wish you good luck , but you won't need it!

Sue Wells

Hi Patsy -

I use chemicon [a division of serologicals] #S7101.

It's a little pricey, but works well. I am willing to share my optimized
protocol with you if you choose this one. I do it several times a month for
various investigators. Most have no trouble distinguishing necrosis from
apoptosis... They look for nuclear "blebbing" and fragmented nuclei, etc.
Most requests come from people who have been doing this a long time. [I'm
glad I don't have to do this part] Please let me know if you have any

Best Regards,

Mary Vaughan HT (ASCP)

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
wk email pruegg@ihctech.net
web site www.ihctech.net  

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